贲门癌中长链非编码RNA和微小RNA差异表达谱及相关性分析
Differential expression profiles and correlation analysis of lncRNA and miRNA in gastric cardia adenocarcinoma

目的:分析贲门癌与癌旁组织中长链非编码RNA(lncRNA)和微小RNA(miRNA)的差异表达谱及二者的相关性。方法:利用基因芯片技术鉴定15例贲门癌与癌旁组织中的lncRNA及miRNA的差异表达谱,生物信息方法分析与H19相互作用的候选miRNA分子,qRT-PCR验证H19和miR-148a-3p在贲门癌中表达的相关性。结果:15例贲门癌与癌旁组织中鉴定出5倍以上差异性表达的lncRNA 182个,2倍以上差异性表达的miRNA 152个。生物信息学预测结果表明,lncRNA H19与7个miRNA表达具有相关性。qRT-PCR验证37例贲门癌与癌旁组织中H19和miR-148a-3p的表达呈负相关(r=-0.398,P<0.001)。结论:贲门癌中差异表达的lncRNA和miRNA分子参与了癌变过程,H19可能直接调控miR-148a-3p的表达。
Aim: To analyze the expression profiles and correlations of long non-coding RNA(lncRNA)and microRNA(miRNA)in gastric cardia adenocarcinoma(GCA)and surrounding tissue.Methods: Using microarray technology, the expression of lncRNA and miRNA in 15 pairs of GCA and matched surrounding mucosa tissue samples were profiled. The correlations between the expressions of lncRNA and miRNA were evaluated by bioinformatics approach, followed by qRT-PCR to verify the correlations.Results: A total of lncRNAs(>5-fold difference)and 152 miRNAs(>2-fold difference)were identified in 15 GCA samples compared with their counterparts. Among these 152 differential miRNAs, 7 miRNA candidates were predicted to be targets of lncRNA H19. In another independent cohort analysis of 37 GCA cases, there was negative correlation between expression of H19 and miR-148a-3p validated by qRT-PCR(r=-0.398, P<0.001).Conclusion: Differentially expressed lncRNAs and miRNAs are implicated in carcinogenesis of gastric cardia. LncRNA H19 may directly modulate the expression of miR-148a-3p