新基因AY358935对B16细胞小鼠体内外生长的影响
Effect of a new gene AY358935 on growth of mouse melanoma B16 cells in vivo and in vitro

目的:探讨新基因AY358935对小鼠黑色素瘤B16在细胞小鼠体内外生长的影响及其可能的机制。方法:构建小鼠AY358935基因真核表达质粒并稳定转染B16细胞。MTT法检测AY358935转染细胞(B16-mAY细胞)、空质粒pcDNA3.1转染细胞(B16-pcDNA3.1细胞)及未转染的B16细胞的体外增殖活性。将B16-mAY及B16-pcDNA3.1细胞分别腹背皮下接种于C57小鼠,观察两组成瘤情况。MTT法检测B16-mAY细胞培养上清对人脐静脉内皮细胞(HUVEC)增殖的影响。结果:B16-mAY细胞的体外增殖活性明显高于B16-pcDNA3.1细胞及对照B16细胞(P<0.05)。B16-mAY细胞移植瘤体积较B16-pcDNA3.1组明显增大(P<0.05)。B16-mAY细胞培养上清组HUVEC增殖活性明显高于B16-pcDNA3.1培养上清组(P<0.05)。结论:AY358935具有促进B16细胞生长的作用,其机制可能涉及促血管生长。
Aim: To explore the effect of a new gene AY358935 on the growth of mouse melanoma B16 cells and its possible mechanism.Methods: The eukaryotic expression plasmid of AY358935 was created and stably transfected to the B16 cells. The growth of the B16 cells transfected with the AY358935(B16-mAY cells), with the pcDNA3.1 plasmid(B16-pcDNA3.1 cells)and the untransfected B16 cells was detected by MTT method.The B16-mAY cells and the B16-pcDNA3.1 cells were inoculated to the C57 mice through the way of back-belly subcutaneous inoculation, and the tumor volume were detected.The effect of the B16-mAY cells cultured supernatant on the growth of human umbilical vein endothelial cells(HUVEC)were detected by MTT method.Results: The proliferation activity of B16-mAY cells in vitro were significantly faster than those of B16-pcDNA3.1 cells and B16 cells(P<0.05). The tumor volume of the mice inoculated with B16-mAY cells were significantly bigger than that of B16-pcDNA3.1 cells group(P<0.05). The proliferation activity of HUVEC cultivated in cultured supernatant of B16-mAY cells was significantly higher than B16-pcDNA3.1 cells(P<0.05).Conclusion: AY358935 can promote the growth of B16 cells, and the mechanism may be involved in promoting the growth of blood vessel