稳定表达荧光素酶基因的TC-1细胞系的构建
Establishment of luciferase-labeled TC-1 cells

目的:通过表达荧光素酶的慢病毒载体感染TC-1细胞株,建立稳定表达荧光素酶的TC-1细胞株。方法:根据荧光素酶基因设计引物,PCR扩增获得目的基因,构建表达荧光素酶的慢病毒载体并包装重组慢病毒,感染TC-1细胞。嘌呤霉素加压筛选,鉴定。用双荧光素酶报告基因检测试剂盒及体外成像系统进行荧光素酶活性检测。结果:经检测证实细胞感染及筛选后,成功构建了稳定高表达荧光素酶的TC-1细胞。结论:获得荧光素酶稳定表达的TC-1细胞,转染效果确切,荧光素酶基因表达稳定。
Aim: To establish luciferase-labeled TC-1 cells.Methods: TC-1 cells were infected by lentivirus packaged by recombinant lentiviral vectors containing luciferase gene. Puromycin was used to filter the cells. The expressing of luciferase in TC-1-luc cells was detected by immunofluorescence imaging and in vivo imaging system.Results: After cell infection and screening, it was shown that a stable TC-1 cell line stably expressing luciferase had been built.Conclusion: TC-1 cell line stably expressing luciferase has been obtained