miR-210基因表达和多态性与肺癌易感性的关系
Relationship of miR-210 gene expression and polymorphism to the lung cancer susceptibility

目的:探讨miR-210基因表达和多态性与肺癌易感性的关系。方法:选择137例原发性肺癌患者为肺癌组,141名体检健康人为对照组。采集研究对象外周血,分离血浆和血细胞并提取白细胞DNA,实时荧光定量PCR法检测血浆miR-210表达水平,基质辅助激光解吸电离飞行时间质谱技术检测miR-210基因多态性。结果:肺癌组血浆miR-210表达水平高于对照组(P<0.05),两组miR-210 rs11246190基因型分布差异有统计学意义(P<0.05)。Logistic回归分析结果显示,引起肺癌发病风险升高的因素有吸烟(OR=2.495,95%CI=1.205～5.168)、血浆miR-210表达水平(OR=1.123,95%CI=1.002～1.259)和rs11246190位点AA基因型(OR=2.479,95%CI=1.271～4.835)。结论: 吸烟、血浆miR-210表达水平及rs11246190位点AA基因型与肺癌易感性有关。
Aim:To explore the relatiouship of miR-210 gene expression and polymorphism to the lung cancer susceptibility.Methods:A total of 137 patients with primary lung cancer were recruited as lung cancer group,and 141 health individuals,as control group. Peripheral blood of the subjects was collected, plasma and blood cells were separated, and peripheral blood leukocyte DNA was extracted. The expression of plasma miR-210 was examined using SYBR Green-based qPCR. Matrix-assisted laser desorption/lonization time of flight mass spectrometry was used to detect the polymorphisms of 4 SNPs of miR-210.Results:The plasma expression level of miR-210 in lung cancer group was significantly higher than that of control group(P<0.05). The difference in the genotype distribution of miR-210 rs11246190 between lung cancer group and control group was significant(P<0.05). Logistic regression analysis revealed that the risk factors of lung cancer and their OR(95%CI)were smoking[2.495(1.205-5.168)], overexpression of plasma miR-210[1.123(1.002-1.259)], and AA genotype in rs11246190[2.479(1.271-4.835)].Conclusion:Smoking, overexpression of miR-210, and AA genotype of rs11246190 are related to the lung cancer susceptibility