α-细辛醚对Eca-109细胞凋亡及XIAP、caspase-3表达的影响*
Effect of α-asarone on apoptosis of Eca-109 cells and expressions of XIAP and caspase-3

目的:观察α-细辛醚对人食管癌Eca-109细胞凋亡的影响及XIAP、caspase-3表达的变化,探讨α-细辛醚的可能作用机制。方法:Eca-109 细胞分为5组,分别给予5-氟尿嘧啶(阳性对照组),25、50、100 mg/L α-细辛醚(α-细辛醚低、中、高剂量组)处理,或仅给予等体积的培养液(空白对照组)。培养48 h后,行AO/EB染色,荧光倒置显微镜下观察细胞形态,用MTT法、Annexin-V-PI法分别检测细胞增殖和凋亡,用Western blot、实时荧光定量PCR分别检测空白对照组、α-细辛醚中剂量组及高剂量组XIAP、caspase-3 mRNA及蛋白的表达水平。结果:与空白对照组比较,α-细辛醚低、中、高剂量组及阳性对照组细胞的增殖率降低、凋亡率升高(P均＜0.05)。与空白对照组比较,α-细辛醚中、高剂量组XIAP mRNA及蛋白的表达均降低,caspase-3 mRNA及蛋白的表达均升高(P均＜0.05)。结论:α-细辛醚可下调XIAP的表达、上调caspase-3的表达,从而抑制Eca-109细胞增殖、促进其凋亡。
Aim: To observe the apoptosis-inducing effect of α-asarone on esophageal carcinoma Eca-109 cells and changes of the expressions of XIAP and caspase-3, and explore the underlying mechanism.Methods: Eca-109 cells were allocated into 5 groups, and treated with 5-FU(positive control group), 25,50,100 mg/L α-asarone(low-,medium-,and high- dose α-asarone groups)and culture medium(blank control group),respectively. After 48 h, morphologic changes were observed under inverted fluorescence microscope after AO/EB staining. MTT and Annexin-V/PI method were used to detect cell proliferation and apoptosis. Western blot and real-time quantitative PCR were adopted to detect XIAP and caspase-3 mRNA and protein expressions in blank control group,medium-dose α-asarone group and high-dose α-asarone group.Results: Compared with blank control group, cell proliferation rate in different doses of α-asarone groups and positive control group decreased significantly, while cell apoptosis rate increased significantly(P＜0.05).In contrast with blank control group,the expressions of XIAP mRNA and protein decreased significantly, while those of caspase-3 increased significantly in medium- and high- dose α-asarone groups(P＜0.05).Conclusion: α-asarone could inhibit Eca-109 cells proliferation and induce cell apoptosis by down-regulating XIAP expression and up-regulating caspase-3 expression