人脑胶质瘤组织中CPEB4的表达及与细胞迁移和侵袭的关系*
Expression of CPEB4 in human brain glioma tissue and its relationship with cell invasion and migration

目的:探讨CPEB4在人脑胶质瘤组织中的表达及其与细胞迁移和侵袭能力的关系。方法:收集30例人脑胶质瘤患者的新鲜胶质瘤组织和瘤旁正常组织标本,应用Western blot法检测CPEB4的表达; 分析CPEB4表达与患者临床病理特征的关系; 分别应用siRNA基因干扰系统和质粒过表达系统下调和上调人脑胶质瘤细胞SKMG-4和T98中CPEB4表达; 应用Transwell实验检测细胞的侵袭和迁移能力; 应用Western blot检测差异表达CPEB4后MMP2和MMP9的表达。结果:30例患者中,22例患者的胶质瘤组织中CPEB4表达高于瘤旁正常组织; CPEB4在脑胶质瘤组织中的高表达与KPS评分和病理分级有关(P＜0.05); 下调SKMG-4细胞CPEB4表达后,siCPEB4#2组迁移细胞数和侵袭细胞数均高于siControl组(P＜0.001),且MMP2和MMP9表达降低; 上调T98细胞CPEB4表达后,CPEB4过表达组迁移细胞数和侵袭细胞数均低于对照组(P＜0.05),且MMP2和MMP9表达增高。结论:CPEB4在脑胶质瘤组织中呈高表达; CPEB4可通过调控MMP2和MMP9表达影响脑胶质瘤细胞的侵袭和迁移能力。
Aim: To explore the expression of CPEB4 in human brain glioma tissue and its relationship with cell invasion and migration.Methods: A total of 30 paired fresh tissue were obtained from the resection specimens of glioma patients. The expression of CPEB4 in tumor and adjacent normal tissue was detected by Western blot. CPEB4 expression was upregulated and downregulated by overexpressing plasmid and siRNA in glioma cell lines SKMG-4 and T98. The cell invasion and migration was detected by Transwell assay.MMP2 and MMP9 expressions were detected by Western blot.Results: The expression of CPEB4 was higher in 22 cases of tumor tissue than that in adjacent normal brain tissue. CPEB4 expression was related with pathological grade and KPS score(P＜0.05).When downregulating CPEB4 expression in SKMG-4 cells by siRNA, pierced cells in siCPEB4#2 group were significantly less than those in siControl group(P＜0.001), and the expressions of MMP2 and MMP9 were also reduced. When upregulating CPEB4 expression in T98 cells by pcDNA-3.1 plasmid, pierced cells in control group were significantly more than those in pcDNA-3.1-CPEB4 group(P＜0.05), and the expressions of MMP2 and MMP9 were also increased.Conclusion: The expression of CPEB4 is higher in brain glioma tissue. CPEB4 could regulate glioma cell invasion and migration ability by regulating the expression of MMP2 and MMP9