心力衰竭小鼠心肌组织中4.1R蛋白的表达*
Expression of 4.1R in myocardial tissue from mice with heart failure

摘要目的：探讨心力衰竭（心衰）小鼠心肌组织中4.1R蛋白的表达及其与心功能的关系。方法：42只雄性健康小鼠随机分为心衰组（n=22）和对照组（n=20）。处死小鼠后迅速取出心肌组织，行HE染色，计算左室质量指数和心肌病理积分；并采用免疫组化染色及免疫荧光标记法检测心衰小鼠心肌组织4.1R蛋白的表达。结果：与对照组比较，心衰组左室质量指数和心肌病理积分升高（t=190.668，Z=31.915，P均<0.05）；心衰组心肌组织4.1R蛋白阳性细胞百分比高于对照组（t=18.607，P=0.001）。Pearson直线相关分析显示，心衰组心肌组织4.1R蛋白阳性细胞百分比与左室质量指数呈正相关（r=0.766，P<0.001）。结论：心衰小鼠心肌组织中4.1R蛋白的表达增加，干预4.1R蛋白的表达可能对心衰的治疗及预后有一定的意义。
AbstractAim: To detect the expression of 4.1R in myocardial tissue from mice with heart failure(HF) and its relationship with cardiac function. Methods: A total of 42 male mice were randomly allocated into HF group (n=22) and control group (n=20). All the mice were killed and the myocardial tissue was prepared for HE staining. The left ventricular mass index and the myocardial tissue pathological score were assessed. The expression of 4.1R in myocardial tissue was detected by immunohistochemistry and immunofluorescence. Results: Compared with control group, the left ventricular mass index and the myocardial tissue pathological score in the HF group increased (t=190.668,Z=31.915,P<0.05). The positive rate of 4.1R in the HF group was significantly higher than that in control group(t=18.607, P=0.001). In the HF group, the positive correlation was found between the left ventricular mass index and the positive rate of 4.1R in myocardial tissue(r=0.766，P<0.001).Conclusion: The expression of 4.1R in myocardial tissue from mice with HR is increased and intervention of the expression of 4.1R may be helpful in the treatment and prognosis of HR