螺内酯对心肌肥厚大鼠心肌组织中可溶性ST2表达的影响
Effects of spironolactone on soluble ST2 in myocardial tissue of hypertrophy rats

目的:研究螺内酯对心肌肥厚大鼠IL-33/ST2通路中可溶性ST2(sST2)的影响。方法:选取健康雄性SD大鼠30只,随机分为对照组(n=10)、模型组(n=10)、药物干预组(n=10)。对照组皮下注射生理盐水,模型组皮下注射异丙肾上腺素(ISO),药物干预组皮下注射ISO+螺内酯20 mg/(kg？d)灌胃,余各组用等量生理盐水灌胃。14 d后处死大鼠,测量心脏质量指数、左心室质量指数及心肌细胞直径; 免疫组化法及RT-PCR法检测心肌组织中sST2的表达。结果:模型组与药物干预组心脏质量指数、左心室质量指数及心肌细胞直径均高于对照组(P<0.05); 模型组sST2的表达高于对照组和药物干预组(P<0.05)。结论:螺内酯可能通过调节IL-33/ST2通路中sST2的表达,抑制ISO诱导的心肌肥厚。
Aim: To study the effects of spironolactone on sST2 of IL-33/ST2 pathway in myocardial hypertrophy rats.Methods: A total of 30 healthy male SD rats were randomly allocated into control group(n=10), model group(n=10)and drug intervention group(n=10).LVM/BM,HM/BM and the size of cardiomyocyte were detected.sST2 mRNA and protein in left ventricular tissue were analyzed by RT-PCR and immunohistochemistry.Results: LVM/BM and HM/BM in the drug intervention group and model group were higher than those in control group(P<0.05).The expression of sST2 in the model group was higher than that in the control group and drug intervention group(P<0.05).Conclusion: Spironolactone could modulate the expression of sST2 in IL-33/ST2 pathway, and alleviate myocardial hypertrophy caused by isoproterenol