结核分枝杆菌Rv1242蛋白抗原表位的预测*
Prediction of epitopes of Mycobacterium tuberculosis Rv1242 protein

目的:预测结核分枝杆菌Rv1242蛋白的抗原表位。方法:利用DNAStar软件包中Protean软件对Rv1242氨基酸序列进行分析,采用包括二级结构、亲水性、抗原性、表面可能性、柔韧性等多参数预测其二级结构及T细胞和B细胞抗原表位。结果:Rv1242蛋白具有丰富的二级结构和多处抗原指数较高的区段,有7个亲水性区域,含有B细胞抗原肽表位(可能在17-38、72-82、84-90、116-130、134-143位氨基酸残基或其附近),这些表位抗原性较好,都含有β转角和不规则卷曲结构,表面可能性和柔韧性都较大。该蛋白还含有T细胞抗原肽表位(可能在24-33、42-59、68-77、90-108位氨基酸残基或其附近)。结论:Rv1242蛋白可能是一个既有T细胞抗原表位、也有B细胞抗原表位的抗原,该研究为进一步研究该蛋白抗原表位及其应用奠定了基础。
Aim: To predict the epitopes of Mycobacterium tuberculosis Rv1242 protein.Methods: The amino acid sequence of Rv1242 protein was input and B cell and T cell epitopes were predicted using multi-parameters such as hydrophilicity,antigenicity,accessibility,flexibility,as well as the secondary structure by Protean software of DNAStar software package.Results: Rv1242 protein had rich secondary structure and multiple sections with higher antigenicity. There were 7 hydrophilic regions. There were potential B cell epitopes at 17-38,72-82,84-90,116-130,134-143 amino acid residues or nearby. These epitopes had better antigenicity, contained beta angle and irregular coil structure, and presented in the surface with larger probability and flexibility. There were also potential T cell epitopes at 24-33,42-59,68-77,90-108 amino acid residues or nearby.Conclusion: Mycobacterium tuberculosis Rv1242 is probably a protein antigen with both T and B cell epitopes, which will lay the foundation for its further study and application