葛根芩连汤对慢性牙周炎合并动脉粥样硬化大鼠TNF？拨了？平的影响*
Effects of Gegenqinlian decoction on TNF？拨？ level in rats with chronic periodontitis and atherosclerosis

摘要目的：探讨葛根芩连汤对牙周炎合并动脉粥样硬化大鼠模型TNF？拨了？平的影响。方法：将40只健康雄性Wistar大鼠分为对照组（N组）和模型组（D组），D组接受造模处理。造模成功后将D组分为盐水治疗组（D1组）、甲硝唑治疗组（D2组）、阿托伐他汀治疗组（D3组）、甲硝唑联合阿托伐他汀治疗组（D4组）、葛根芩连汤治疗组（D5组），灌胃4周后，HE染色光镜下观察牙周组织和腹主动脉病理变化，采用免疫组化法检测TNF？拨猎谘乐茏橹？和腹主动脉中的表达，采用ELISA法检测血清TNF？拨了？平。结果：D2、D3、D4、D5组牙周组织和腹主动脉中TNF？拨料喽员泶锪烤？较D1组降低（P均<0.05）。 D2、D3、D4、D5组血清中TNF？拨了？平均较D1组降低（P<0.05）。结论：葛根芩连汤能降低牙周炎合并动脉粥样硬化大鼠TNF？拨了？平，对牙周炎伴动脉粥样硬化疾病有良好的治疗效果。
AbstractAim: To observe the effects of Gegenqinlian decoction on TNF？拨？ level in rats with periodontitis and atherosclerosis. Methods: A total of 40 healthy male Wistar rats were divided into normal control group(N group) and model group(D group). After successful modeling, D group was divided into saline？？treated group(D1 group), metronidazole treatment group(D2 group), atorvastatin treatment group(D3 group), metronidazole plus atorvastatin treatment group(D4 group), and Gegenqinlian decoction treatment group(D5 group). Immunohistochemical staining was used to detect TNF？拨？ in periodontal tissue and abdominal aorta. ELISA was used to detect serum TNF？拨？ level. Results: TNF？拨？ level in D2, D3, D4, D5 group was lower compared with D1 group, and the difference was statistically significant(P<0.05). Conclusion: Gegenqinlian decoction could reduce TNF？拨？ level in rats with periodontitis and atherosclerosis