沉默NOX1表达对A549细胞增殖的影响*
Effect of silencing NOX1 on proliferation of A549 cells

目的:探讨NOX1对肺癌A549细胞凋亡和增殖的影响。方法:通过瞬时转染技术转染特异性的NOX1小干扰RNA(siRNA),按转染情况分为Mock组、NC组、FAM-NC-NOX1 siRNA组,转染12 h后采用Western blot检测NOX1蛋白的表达,通过荧光探针DCFH-DA检测细胞ROS水平,流式细胞仪检测细胞增殖活性和凋亡率。结果:siRNA转染12 h以上,转染率稳定在80%以上,NOX1蛋白的表达量与Mock组相比下降(P<0.05); 与Mock组相比, FAM-NC-NOX1 siRNA组细胞在24、36、48 h的增殖活性均下降,ROS水平也下降(P<0.05)。结论:NOX1 siRNA转染A549细胞能有效抑制细胞的增殖。
Aim: To explore the proliferation and apoptosis in A549 cells after down-regulating NOX1 expression.Methods: NOX1 interference in A549 cells was achieved by NOX1 small interference RNA(siRNA). A549 cells were allocated into Mock group, NC group, and FAM-NC-NOX1 siRNA group. Being transfected specifically through the transient transfection technology about 12 h,the expression of NOX1 protein was detected by Western blot,then the fluorescent probe DCFH-DA was used to detect the ROS levels of cells,the flow cytometry was used to detect the changes of cell proliferation activity and apoptosis.Results: The transfection efficiency of NOX1 siRNA was above 80% in A549 cells. Compared with the Mock group,NOX1 protein expression was decreased, the cell viability was decreased at 24, 36, 48 h after transfection,and the ROS level in FAM-NC-NOX1 siRNA group was decreased(P<0.05).Conclusion: NOX siRNA transfection could effectively inhibit the proliferation of A549 cells