食管鳞癌组织中GSK3β表达的临床意义
Clinical significance of GSK3β expression in esophageal squamous cell carcinoma tissue

目的:分析GSK3β在食管鳞癌组织中的表达及临床意义。方法:采用实时荧光定量PCR、Western blot、免疫组化等方法检测GSK3β在52例食管鳞癌及相应癌旁组织中的表达,并采用Cox回归分析GSK3β表达与食管鳞癌患者术后预后的关系。结果:食管鳞癌组织中GSK3β mRNA及蛋白表达水平、阳性表达率均高于相应癌旁组织(P<0.05)。Cox回归分析表明GSK3β为食管鳞癌患者术后预后的独立危险因素(β=1.095,P=0.026)。结论:GSK3β表达可能促进了食管鳞癌的发生发展; GSK3β可能成为食管鳞癌靶向治疗的分子靶点之一。
Aim: To characterize the expression level of GSK3β and its clinical significance in esophageal squamous cell carcinoma(ESCC)tissue.Methods: The mRNA and protein expression levels of GSK3β in 52 cases of ESCC and matched adjacent tissue samples were determined by Real-time PCR, Western blot and immunohistochemistry, respectively, and the correlation between the expression of GSK3β and the prognosis of patients was also analyzed using Cox regression.Results: The mRNA and protein expression levels, and the positive rate of GSK3β in ESCC tissue were higher compared with those in adjacent noncancerous mucosa tissue(P<0.05). Cox regression analysis showed that GSK3β was an independent risk factor for the prognosis of patients with ESCC(β=1.095,P=0.026).Conclusion: GSK3β expression may promote the development of ESCC, and GSK3β may be one of the molecular targets for treatment of ESCC