SP-D基因SNP与河南汉族人群肺结核相关性分析
Relationship between SNP of SP？？D and pulmonary tuberculosis in Henan Han population

目的：检测河南汉族人群中SP？？D11和SP？？D160位点单核苷酸多态性（SNP）与肺结核易感性之间的关系。方法：用SSP？？PCR、琼脂糖凝胶电泳和基因测序的方法检测河南汉族197例肺结核患者和200例正常人的SP？？D11、SP？？D160位点的SNP，并进行基因型频率、等位基因频率的比较。结果：SP？？D11位点CT基因型频率在肺结核组和对照组间差异有统计学意义［P=0.006，OR(95%CI)=1.932（1.196~3.122）］；SP？？D160位点A等位基因频率在45岁以下人群肺结核组和对照组间差异有统计学意义［P=0.028，OR(95%CI)=1.524（1.044~2.226）］。结论：SP？？D11位点CT基因型、SP？？D160位点A等位基因与河南汉族人群肺结核发病有关，且可能受到年龄的影响。
Aim: To study the relation between single nucleotide polymorphism at SP？？D11 and SP？？D160 and pulmonary tuberculosis(TB) of Han population in Henan Province. Methods: A total of 197 cases of TB and 200 healthy subjects were recruited in the study. SSP？？PCR, agarose gel electrophoresis and gene sequencing analysis method were used to detect genotype of SP？？D gene on 11, 160 sites. Results: CT genotype frequency on SP？？D11 site had statistical difference between TB group and control group［P=0.006，OR(95%CI)=1.932（1.196-3.122）］.In ＜45 years old group, A allele frequency on SP？？D160 site had statistical difference between TB group and control group［P=0.028，OR(95%CI)=1.524（1.044-2.226）］.Conclusion: CT genotype on SP？？D11 site and allele A on SP？？D160 loci are associated with TB in Henan Han population, which may be influenced by age