肾血管性高血压大鼠肾组织睾酮受体蛋白及丝裂素活化蛋白激酶磷酸酶1的表达
Expressions of androgen receptor and mitogen-activated protein kinase phosphatase-1 in rats with renovascular hypertension

目的:探讨睾酮受体(AR)在高血压肾脏损害中的作用并初步探讨其机制。方法:30只大鼠分为对照组、假手术组和模型组,每组10只; 模型组以“两肾一夹法”建立肾血管性高血压大鼠模型。采用免疫组化法检测大鼠肾组织AR和丝裂素活化蛋白激酶磷酸酶1(MKP-1)蛋白的表达。结果:与对照组相比,模型组大鼠肾组织AR与MKP-1蛋白的表达水平降低(F=31.043、51.681,P<0.001); 相关分析发现,模型组大鼠肾组织中AR蛋白与MKP-1蛋白的表达呈正相关(r=0.744,P=0.014)。结论:AR蛋白可能参与了高血压肾脏损害的过程,其机制可能与AR和MKP-1信号转导通路偶联有关。
Aim: To explore the role of androgen receptor(AR)in injury of hypertensive kidney and explore the mechanism.Methods: Thirty rats were allocated into 3 groups: control group, sham operation group and model group, with 10 rats in each group; the rats in model group were made renovascular hypertension model. The expressions of AR and mitogen-activated protein kinase phosphatase-1(MKP-1)were detected by immunohistochemistry.Results: In contrast with control group, the protein expression levels of AR and MKP-1 in model group were significantly reduced(F=31.043,51.681,P<0.001). Linear correlation analysis showed there was positive correlation between the expression level of AR protein and that of MKP-1 protein in the model group(r=0.744,P=0.014).Conclusion: AR may participate in the hypertensive renal injury,which may be explained by MKP-1 signaling pathway that couples with AR