五倍子酸对人纤维肉瘤HT1080细胞增殖和凋亡的影响*
Inhibitory effects of gallic acid on human fibrocarcinoma cell HT1080 proliferation and apoptosis

摘要目的：研究五倍子酸（GA）对人纤维肉瘤HT1080细胞增殖和凋亡的影响。方法：分别用3.125、6.250、12.500、25.000、50.000和100.000 mg/L的GA处理HT1080细胞48 h后，应用MTT法检测细胞的抑制率；分别以6.250、12.500和25.000 mg/L的GA作用HT1080细胞48 h后，应用流式细胞仪检测细胞凋亡情况，应用免疫细胞化学方法检测GA对HT1080细胞中Bcl？？2和Bax蛋白表达的影响。结果：GA对HT1080细胞以剂量依赖的方式抑制其增殖（F=71.214，P<0.001)、诱导其凋亡（F=52.217，P<0.001），并能上调Bax蛋白的表达（F=11.024，P<0.001）、下调Bcl？？2蛋白的表达（F=8.741, P<0.001）。结论：GA可能通过上调Bax蛋白的表达和下调Bcl？？2蛋白的表达来抑制HT1080细胞增殖和诱导其凋亡。
AbstractAim: To explore the inhibitory effects of gallic acid(GA) on human fibrocarcinoma cell HT1080. Methods: MTT was employed to detect proliferative ability in HT1080 cells treated with 3.125, 6.250, 12.500, 25.000, 50.000 and 100.000 mg/L GA for 48 h, respectively. Flow cytometry was used to examine apoptotic rate in HT1080 cells treated with 6.250, 12.500 and 25.000 mg/L GA for 48 h, respectively. Immunocytochemistry was applied to explore the change of Bcl？？2 and Bax expression in HT1080 cells.Results: The proliferation inhibition rate of HT1080 cells was enhanced in a dose？？dependent manner（F=71.214，P<0.001). The number of apoptosis cell increased in a dose？？dependent manner(F=52.217, P<0.001). GA up？？regulated Bax expression（F=11.024，P<0.001） and down？？regulated Bcl？？2 expression（F=8.741, P<0.001）. Conclusion: GA probably inhibit the proliferation and induce apoptosis of HT1080 cells by up？？regulating Bax and down？？regulating Bcl？？2