表皮生长因子受体在臭氧致小鼠肺部炎症中的作用
Role of EGF receptor in ozone-induced lung inflammation in mice

目的:探讨表皮生长因子受体(EGFR)在臭氧致小鼠肺部炎症中的作用。方法:40只SPF级BALB/c小鼠随机分为4组(n=10),分别暴露于空气或0.5、1.0、2.0 mg/m3臭氧中,每天暴露3 h,连续暴露7 d。HE染色观察小鼠肺部炎症病理学改变,并进行评分; 免疫组化法检测小鼠支气管上皮EGFR(Y1068)磷酸化水平; 双抗夹心ELISA法测定支气管肺泡灌洗液(BALF)中趋化因子CXCL1的含量。结果:0.5、1.0、2.0 mg/m3臭氧组小鼠肺组织炎症评分均高于空气组(P均＜0.05),小鼠支气管上皮EGFR(Y1068)磷酸化水平和BALF中趋化因子CXCL1的表达水平亦均高于空气组(P均＜0.05)。相关分析结果显示,小鼠支气管上皮EGFR(Y1068)磷酸化水平与小鼠肺组织炎症病理学变化总分(r=0.652, P=0.030)、BALF中趋化因子CXCL1表达水平(r=0.925, P＜0.001)均呈正相关。结论:EGFR磷酸化可能与臭氧诱导的小鼠肺部炎症有关。
Aim: To explore the role of epidermal growth factor receptor(EGFR)in ozone-induced lung inflammation in mice.Methods: Forty BALB/c mice were randomly allocated into four groups(n=10), and then exposed to air or 0.5, 1.0, 2.0 mg/m3 ozone(3 h/d for 7 d), respectively. Inflammatory and pathological alterations were observed by HE staining. Phosphorylation of EGFR(Y1068)in bronchial epithelium was examined using immunohistochemical staining. Level of CXCL1 expression in bronchoalveolar lavage fluid(BALF)of mice was measured using ELISA.Results: Compared with air-exposed mice, scores of inflammatory and pathological alterations were increased after exposure to 0.5, 1.0, 2.0 mg/m3 ozone(P＜0.05). 0.5, 1.0, 2.0 mg/m3 ozone exposure elevated levels of phosphorylated EGFR(Y1068)in bronchial epithelium(P＜0.05). In addition, level of CXCL1 expression in BALF of 0.5, 1.0, 2.0 mg/m3 ozone-exposed mice was increased(P＜0.05). There were positive correlation between level of phosphorylated EGFR(Y1068)and total score of inflammatory and pathological alterations(r=0.652, P=0.030), CXCL1 expression in BALF(r=0.925, P＜0.001).Conclusion: Phosphorylation of EGFR(Y1068)in bronchial epithelium is related to ozone-induced lung inflammation in mice