脂肪间充质干细胞治疗实验性自身免疫性脑脊髓炎小鼠的效果*
Efficacy of adipose mesenchymal stem cell on experimental autoimmune encephalomyelitis mice

目的:观察脂肪间充质干细胞(ADMSC)移植治疗实验性自身免疫性脑脊髓炎(EAE)小鼠的疗效。方法:实验第0、7天采用髓鞘少突胶质细胞糖蛋白作为抗原与完全弗氏佐剂免疫小鼠,ADMSC组(n=14)小鼠于免疫后第14、21、28天尾静脉注射ADMSC(5.5×106个/只),EAE组(n=13)注射同等体积的生理盐水; 对照组(n=12)不免疫,不给予ADMSC。实验过程中对小鼠进行神经功能缺损评分; 第33天,取脊髓行病理观察,进行炎性细胞浸润评分和脱髓鞘评分; ELISA法检测小鼠外周血TNF-α、IL-17和IL-4水平; 流式细胞术测定小鼠脾脏中CD4+Foxp3+ T细胞(Treg)的比例。结果:与EAE组相比,ADMSC组小鼠神经功能缺损评分、脊髓组织中炎性细胞浸润和脱髓鞘评分降低(P＜0.05); 外周血IL-4水平升高,而TNF-α和IL-17水平降低(P＜0.05); 脾脏中Treg细胞比例明显升高(P＜0.05),接近对照组水平。结论:ADMSC移植可显著改善EAE小鼠的神经功能。
Aim: To observe the efficacy of adipose mesenchymal stem cell(ADMSC)on experimental autoimmune encephalomyelitis(EAE)mice.Methods: At the 0,7th day during the experiment, the mice in EAE group(n=13)and ADMSC group(n=14)were immuned with MOG35-55/CFA emulsion; at the 14th,21st,28th day,ADMSC(5.5×106 cells per mouse)were injected via the tail vein in ADMSC group, while normal saline instead of ADMSC were given in EAE group. The control group(n=12)were not immuned or injectd with ADMSC. Nerve function injury scoring was performed during the experiment. At the 33th day, myelin depigmentation and inflammatory cell infiltration score for spinal cord were carried out, TNF-α,IL-17,and IL-4 level in the peripheral blood were evaluated by ELISA,and the percent of CD4+Foxp3+ Treg cells were analyzed by flow cytometry.Results: Compared with those of EAE group, nerve function score, myelin depigmentation score and inflammatory cell infiltration score of the ADMSC group significantly decreased(P＜0.05); the level of IL-4 increased, while the levels of IL-17 and TNF-α decreased(P＜0.05); the percent of CD4+Foxp3+ Treg cells significantly increased(P＜0.05)and reached to the level of the control group.Conclusion: ADMSC transplantation could effectively improve neural function in experimental autoimmune encephalomyelitis mice