4.1R基因敲除小鼠血液生理生化参数分析禹丽娜,李建辉,张丽果,宁姝威,薛超越,
Analysis on blood physiological and biochemical parameters of 4.1R gene knockout mice

目的:检测并分析蛋白4.1R基因敲除小鼠(4.1R-/-)和野生型小鼠(4.1R+/+)血液生理生化参数。方法:摘眼球法采集两组小鼠(n=6)血液,用全自动血液细胞分析仪和生化分析仪检测血液生理生化参数。结果:血液生理参数中,4.1R-/-小鼠红细胞计数、血红蛋白、红细胞压积和中性粒细胞比率显著低于野生型小鼠(P＜0.05); 血小板计数、平均血小板体积、血小板压积、白细胞计数、淋巴细胞比率显著高于野生型小鼠(P＜0.05)。生化参数中,4.1R-/-小鼠碱性磷酸酶显著低于野生型小鼠,总胆红素、直接胆红素和间接胆红素显著高于野生型小鼠(P＜0.05)。结论:蛋白4.1R缺失可导致慢性溶血性贫血,4.1R-/-小鼠相关血液生理生化参数随之改变。
Aim: To detect and analyze blood physiological and biochemical parameters of 4.1R gene knockout mice(4.1R-/-)and wild-type(4.1R+/+)mice.Methods: Blood samples of 4.1R-/- mice and wild-type mice were collected by enucleating of eyeball, followed by blood physiological and biochemical parameters testing with automatic blood cell analyzer and biochemical analyzer.Results: Compared with those of the wild-type mice,RBC, HGB, HCT,and NEU of 4.1R-/- mice were obviously lower(P＜0.05); while PLT,MPV,PCT,WBC and LYM of 4.1R-/- mice were obviously higher(P＜0.05). ALP,one of biochemical parameters, was significantly lower,while T-BIL, D-BIL and I-BIL of 4.1R-/- mice were significantly higher(P＜0.05).Conclusion: Deletion of protein 4.1R might result in chronic hemolytic anemia, and changes of blood physiological and biochemical parameters may be basic information for the further study with 4.1R-/- mice model