β-胡萝卜素对食管鳞癌EC1细胞内活性氧和细胞凋亡的影响
The effect of β-carotene on the intercellular ROS level and apoptosis of esophageal squamous cell carcinoma EC1 cell

目的:探讨β-胡萝卜素对食管鳞癌EC1细胞内活性氧和细胞凋亡的影响。方法:分别用不同质量浓度的β-胡萝卜素(30、50、80 μmoL/L)处理食管鳞癌EC1细胞 48 h后,采用化学荧光法检测EC1细胞内活性氧(ROS)含量变化,通过JC-1从红色荧光到绿色荧光的转变检测线粒体膜电位(MMP)变化,ELISA法检测胞内细胞色素C(Cyt-C)含量的变化,Western blot检测Bax、Bcl-2和Caspase-3蛋白的表达,流式细胞术检测不同质量浓度的β-胡萝卜素对EC1细胞凋亡的影响。结果:β-胡萝卜素处理能够提高EC1细胞内ROS含量,引起MMP下降,促进Cyt-C的释放,上调Bax和Caspase-3蛋白表达,并下调Bcl-2蛋白表达; 同时,β-胡萝卜素作用能提高EC1细胞凋亡率。结论:β-胡萝卜素能够通过上调细胞内ROS的水平,降低MMP,进一步促进食管鳞癌细胞凋亡。
Aim: To study the effect of β-carotene on the intercellular ROS level and apoptosis of esophageal squamous cell carcinoma EC1 cell.Methods: EC1 cells were treated with different concentrations of β-carotene(30, 50, 80 μmoL/L)for 48 h. The content of reactive oxygen species(ROS)in EC1 cells was detected by chemiluminescence method. The change of mitochondrial membrane potential was detected by the conversion of JC-1 from red fluorescence to green fluorescence. ELISA was used to detect the content of cytochrome C(Cyt-C). The expression of Bax, Bcl-2 and Caspase-3 proteins was detected by Western blot. Flow cytometry was used to detect the effects of different concentrations of β-carotene on apoptosis of EC1 cells.Results: β-carotene treatment significantly increased ROS content in EC1 cells, decreased mitochondrial membrane potential, and promoted release of Cyt-C. The expression of Bax and Caspase-3 proteins was up-regulated and Bcl-2 protein expression was down-regulated. Meanwhile, β-carotene can significantly increase the rate of apoptosis of EC1 cells.Conclusion: β-carotene can promote the apoptosis of esophageal squamous cell carcinoma by up-regulating the expression of intracellular ROS and decreasing the mitochondrial membrane potential