乳癌患者转移淋巴结中T细胞受体β链互补决定区3谱型分析
Analysis of complementarity determining region 3 repertoire of T cell receptor β chain in metastatic lymph node from patients with breast cancer

目的：分析乳癌患者转移淋巴结标本中T细胞受体（TCR）β链互补决定区3（CDR3）的谱型偏移及氨基酸序列特点。方法：选取5例乳癌患者转移淋巴结和2例乳腺反应性增生患者的淋巴结标本，应用RT？？PCR扩增T细胞24个TCR β链可变区（BV）亚家族，经基因扫描确定T细胞克隆性，对提示单、寡克隆性增生的TCR BV亚家族的PCR产物进行测序。结果：乳腺反应性增生患者淋巴结中T细胞均为多克隆性增生。乳癌患者转移淋巴结中T细胞呈单、寡克隆及多克隆性增生，克隆性增生T细胞中存在2～5个BV亚家族的谱型偏移；CDR3氨基酸序列分析证实单、寡克隆性增生T细胞的CDR3氨基酸序列呈现多样性的特点。结论：乳癌患者TCR BV亚家族取用存在谱型偏移，不同T细胞克隆的CDR3氨基酸序列不同。
Aim: To analyze the specific repertoire skewing and amino acid sequences of the complementarity determining region 3(CDR3) of T cell receptor(TCR) β chain in metastatic lymph node from patients with breast cancer. Methods: A total of 24 TCR β chain variable region(BV) subfamilies were amplified by RT？？PCR, and the CDR3 lengths were analyzed with GeneScan method in lymph nodes from 2 patients with reactive hyperplasia and metastatic lymph nodes from 5 patients with breast cancer.Sequence analysis of the CDR3 region in monoclonal or oligoclonal expanded T cells was performed.Results: There were only multiclonal expanded T cells in lymph nodes from patients with reactive hyperplasia, while there were monoclonal, oligoclonal, and multiclonal expanded T cells in metastatic lymph nodes from patients with breast cancer. TCR BV presented specific repertoire skewing in metastatic lymph node from patients with breast cancer, among which only 2？？5 TCR BV subfamilies were identified.The monoclonal or oligoclonal expanded T cells had different CDR3 amino acid sequences. Conclusion: There is specific repertoire skewing of TCR BV subfamilies in metastatic lymph nodes from patients with breast cancer. The amino acid sequences of CDR3 in different T cell clone proliferation are mostly different