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- 2015
小脑顶核通过小脑-下丘脑通路参与卒中后抑郁的实验观察
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Abstract:
摘要：目的 探讨小脑顶核是否参与了卒中后抑郁的发病，并初步研究介导这种作用的途径。方法 选用健康SD大鼠，随机分为假手术组、卒中组、卒中后抑郁组、小脑顶核损毁组和小脑上脚交叉损毁组，进行行为学观察并应用高效液相色谱法检测各组大鼠下丘脑外侧区Glu、GABA的含量。结果 与假手术组相比，卒中组大鼠下丘脑外侧区Glu、GABA的含量无统计学差异(P>0.05)；而与卒中组相比，卒中后抑郁组、小脑顶核损毁组和小脑上脚交叉损毁组下丘脑外侧区Glu、GABA含量都有不同程度的降低(P<0.01)。结论 本实验初步提示小脑顶核可能参与了卒中后抑郁的发病，其途径可能通过小脑-下丘脑通路介导。
ABSTRACT: Objective To investigate whether medial cerebellar nucleus may be involved in the pathogenesis of post-stroke depression (PSD) and explore the pathway that mediates this effect. Methods Healthy SD rats were randomly divided into five groups: control group, stroke group, PSD group, medial cerebellar nucleus (Med) lesion group, and superior cerebellar peduncle (xscp) lesion group. The ethological score of depression was evaluated. The neurotransmitters of Glu and GABA in the lateral hypothalamic area were detected by high performance liquid chromatography. Results Compared with those in control group, the levels of GABA and Glu expressions did not significantly differ in the lateral hypothalamic area in stroke group (P >0.05). In contrast, the levels in PSD group, Med lesion group and xscp lesion group were lower than those in stroke group to different extent (P<0.01). Conclusion These results preliminarily show that medial cerebellar nucleus may participate in the pathogenesis of post-stroke depression, which may be mediated by the cerebellum-hypothalamic pathway
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