适用于细粒棘球蚴囊液蛋白质组分析的双向电泳技术
Two-dimensional electrophoresis for proteomics analysis of hydatid fluid of the Echinococcus granulosus

摘要：目的 建立适用于细粒棘球蚴囊液蛋白质组的双向电泳分离条件。方法 冷冻干燥法制备囊液总蛋白提取物，观察不同的蛋白样品处理方法、IPG胶条pH范围和电泳上样量对双向电泳图谱的影响。结果 经ReadyPrepTM 2-D Cleanup Kit处理的提取物上样量为400μg，在pH7～10范围的胶条进行双向电泳分离，可获取蛋白斑点较多、重复性较好的二维电泳图谱。结论 建立的细粒棘球蚴囊液双向电泳技术及图谱，可为细粒棘球蚴虫蛋白质组学的研究提供理论依据。
ABSTRACT: Objective To establish two-dimensional electrophoresis (2-DE) technology for hydatid fluid of the parasite Echinococcus granulosus. Methods We extracted total proteins of Echinococcus granulosus by lyophilization and studied the changes of 2-DE picture under different methods of dealing with the protein samples, pH range of IPG strip and quantitative loading of the samples. Results The two-dimensional collection of illustrative plate with better resolution and repetition was achieved when the hydatid fluid dealt with ReadyPrepTM 2-D Cleanup Kit was analyzed using 400μg of quantitative loading and IPG strips pH 7-10. Conclusion The established 2-DE method and maps of hydatid fluid of the parasite Echinococcus granulosus can provide theoretical evidence for proteomic study of Echinococcus granulosus