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- 2016
淋巴细胞亚群在不同周龄自发性高血压大鼠和正常大鼠脾脏中的表达差异
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Abstract:
摘要：目的 研究T淋巴细胞亚群在不同周龄的自发性高血压大鼠(spontaneously hypertensive rat, SHR)和正常Wistar京都(Wistar-Kyoto, WKY)大鼠脾脏中的表达差异。方法 选取16周龄和40周龄雄性SHR和WKY大鼠各8只，监测血压后，通过苏木素-伊红(HE)染色观察大鼠脾脏病理学变化，应用流式细胞术检测WKY大鼠和SHR脾脏中T淋巴细胞亚群的表达变化。结果 与相同周龄WKY大鼠比较，SHR脾脏中央动脉血管壁增厚，管腔狭窄，且40周龄SHR脾脏病变较16周龄严重； 相同周龄的SHR与WKY大鼠比较，脾脏中CD3+、CD3+CD4+和CD3+CD8+ T淋巴细胞比例均无统计学差异(P>0.05)；与16周龄WKY大鼠比较，40周龄WKY大鼠脾脏CD3+和CD3+CD8+ T淋巴细胞比例显著降低(P<0.01)，CD3+CD4+ T淋巴细胞比例和CD4+/CD8+比值显著升高(P<0.01)；40周龄SHR脾脏CD3+、CD3+CD4+、CD3+CD8+ T淋巴细胞以及CD4+/CD8+比值的变化趋势同WKY大鼠相一致。结论 高血压可引起脾脏病理改变，但其对脾脏T淋巴细胞亚群比例可能无显著影响，然而随着周龄的增加，脾脏T淋巴细胞亚群均发生紊乱。
ABSTRACT: Objective To investigate the difference in the expression of T lymphocyte subsets in the spleen of spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats at different weeks of ages. Methods Sixteen-week-old and forty-week-old male SHRs and WKY rats (n=8 respectively) were used in this study. After measurement of blood pressure, the pathological changes in spleen tissue were observed by hematoxylin-eosin (HE) staining. And splenic T lymphocyte subsets of WKY rats and SHRs were detected by flow cytometry. Results Compared with age-matched WKY rats, SHRs showed thickening of central artery vascular walls and luminal stenosis; spleen pathology was more serious in forty-week-old SHRs than in sixteen-week-old SHRs. No significant differences were observed in the percentage of CD3+, CD3+CD4+ or CD3+CD8+ T lymphocytes in the spleen of age-matched SHRs and WKY rats (P>0.05). Compared with sixteen-week-old WKY rats, forty-week-old WKY rats had a significantly decreased percentage of CD3+ and CD3+CD8+ T lymphocytes in the spleen (P<0.01), but an increased percentage of CD3+CD4+ T lymphocytes and CD4+/CD8+ ratio in the spleen (P<0.01). The change tendency for CD3+, CD3+CD4+ and CD3+CD8+ T lymphocytes and CD4+/CD8+ ratio in forty-week-old SHRs was consistent with that in the spleen of WKY rats. Conclusion Hypertension can cause pathological changes of the spleen, but it may not significantly affect splenic T lymphocyte subsets. However, aging may disrupt the homeostasis of T lymphocyte subsets
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