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- 2016
MicroRNA-21在急性髓系白血病骨髓细胞中的异常表达及意义
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Abstract:
摘要：目的 分析急性髓系白血病(AML)患者骨髓细胞中miR-21的表达情况及其在白血病发生发展中的可能作用及临床意义。方法 应用实时荧光定量PCR检测40例初治AML患者、22例完全缓解期AML(AML-CR)患者、13例难治复发患者、20例非血液肿瘤患者及健康人骨髓中miR-21的表达水平，并分析AML患者miR-21的表达水平与临床指标的相关性。结果 miR-21在AML中的表达是上调的，初治组、完全缓解组及难治复发组miR-21的表达水平均高于对照组(P<0.05)；完全缓解组miR-21的表达量较初治组有所下降(P<0.05)；难治复发组miR-21的表达量高于初治组，但两组间无统计学意义(P>0.05)；初治AML miR-21表达水平与骨髓原始幼稚细胞数呈正相关(P<0.05)，与外周血白细胞数、血小板计数、血红蛋白含量以及年龄、性别、染色体异常等均无明显相关性(P>0.05)。结论 miR-21在急性髓系白血病患者骨髓细胞中的表达上调，并与肿瘤负荷相关，可能在白血病的发生发展中起重要作用，并有望成为白血病诊断的新的分子标志物。
ABSTRACT: Objective To investigate the expression level of miR-21 in bone marrow mononuclear cells (BMMNCs) of patients with acute myeloid leukemia (AML) and explore its effect and clinical significance in the pathogenesis and development of AML. Methods Real-time quantitative reverse transcription PCR(qRT-PCR) was used to examine the expression level of miR-21 in 75 AML patients, including 40 patients with newly diagnosed AML, 22 patients AML with complete remission (CR), 13 refractory AML patients and 20 cases of normal bone marrow. We analyzed the relationship between miR-21 expression and clinical features of the patients. Results The expression of miR-21 was upregulated in AML, and miR-21 expression was significantly higher in initial treatment group, CR group and refractory recurrent group than in control group (P<0.05). The expression of miR-21 in CR group was lower than in the initial treatment group (P<0.05); it was higher in refractory recurrent group than in the initial treatment group, but without significant difference (P>0.05). The expression of miR-21 after initial treatment had a positive correlation with the number of original na？ve bone marrow cells (P<0.05), but no obvious correlation with the patients？？ peripheral blood leukocyte count, platelet count, hemoglobin content, age, gender, or chromosome abnormality (P>0.05). Conclusion miR-21 is excessively expressed in bone marrow cells of AML and is correlated with patients？？ body tumor load, indicating that miR-21 may be involved in the development of AML and can be used as a new molecular marker to guide clinical treatment
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