胚胎/多能干细胞标记物Oct3/4在乳腺浸润性小叶癌中的表达及其对分子分型的意义
Expression of Oct3/4 in breast invasive lobular carcinoma and its molecular subtype significance

摘要：目的 研究干细胞标记物Oct3/4在乳腺浸润性小叶癌组织中的表达及其对分子分型的意义。方法 回顾性分析67例临床病理和术后随访资料完整的乳腺癌患者，采用免疫组织化学方法检测乳腺浸润性小叶癌及正常乳腺组织中Oct3/4的表达情况，探讨Oct3/4表达与其分子分型及临床病理参数的关系。结果 Oct3/4在癌组织中的阳性率明显高于癌旁正常乳腺组织(P<0.05)；Oct3/4在基底细胞样型、Luminal A型、Luminal B(HER2-)型、Luminal B(HER2+)型、HER2(+)型浸润性小叶癌阳性表达率分别为77.78%、29.63%、14.28%、20%、21.43%，差异有统计学意义(P<0.05)；Oct3/4与CK5/6的表达呈正相关性(r=0.394，P<0.05)；Oct3/4阳性表达与患者年龄、是否绝经、肿瘤大小、腋淋巴结转移情况、AJCC分期、患者生存状况及组织学分型均无相关性。结论 干细胞标志物Oct3/4表达可能与乳腺浸润性小叶癌的发生相关，但与其进展和预后关系不明显；Oct3/4表达与浸润性小叶癌的基底细胞样型存在一定相关性；Oct3/4与CK5/6在分子机制上可能存在一定的联系。
ABSTRACT: Objective To explore the expression of Oct3/4 in the molecular subtype of invasive lobular carcinoma of the breast and its clinicopathological significance. Methods Based on the complete and follow-up clinicopathological data and analysis of 67 cases of invasive lobular carcinoma, the expression of Oct3/4 in invasive lobular carcinoma and normal breast tissues was detected using immunohistochemistry; the relationships among Oct3/4 expression, its molecular subtype and its clinicopathological parameters were analyzed. Results The positive rate of Oct3/4 in carcinoma was significantly higher than that of the adjacent normal breast tissue (P<0.05). The Oct3/4-positive expression rates in basal-like subtype, luminal A subtype, luminal B (HER2-) subtype, luminal B (HER2+) subtype and HER2+ subtype of invasive lobular carcinoma were77.78%, 29.63%, 14.28%, 20% and 21.43%, respectively (P<0.05). The expression of Oct3/4 was positive correlated with that of CK5/6 (r=0.394, P<0.05). The positive expression of Oct3/4 did not necessarily depend on patient’s age, histological type, menopausal status, tumor size, axillary lymph node metastasis, AJCC staging or survival of the patients. Conclusion The expression of Oct3/4 is correlated with the basal-like subtype of invasive lobular carcinoma. The expression of Oct3/4 may be associated with the occurrence of invasive lobular carcinoma, though it may not be applied to the progress and prognosis of invasive lobular carcinoma. There may be certain correlation between Oct3/4 and CK5/6 considering molecular mechanism