黄芩苷对宫颈癌HeLa细胞侵袭转移的抑制作用及其机制
Inhibitory effect of baicalin on invasion of cervical cancer HeLa cells and its mechanism

摘要：目的 观察黄芩苷对宫颈癌HeLa细胞侵袭转移的作用及其相应机制。方法 利用MTT法检测黄芩苷对HeLa细胞增殖的影响；Transwell小室法检测黄芩苷对HeLa细胞侵袭能力的影响；Real-time PCR法检测黄芩苷对MMP-2、MMP-9 RNA表达水平的影响；Western blot法检测黄芩苷对MMP-2、MMP-9蛋白表达的影响；Western blot法检测黄芩苷对P38和p-P38蛋白表达的影响；Real-time PCR法检测黄芩苷和sb203580联合应用对MMP-2和MMP-9 RNA表达水平的影响。结果 黄芩苷能够有效抑制HeLa细胞的增值，当质量浓度超过60μg/mL时，与对照组相比(0μg/mL)差异出现统计学意义。在低于细胞增殖抑制浓度时，黄芩苷也能够有效抑制HeLa细胞的侵袭，10、20、40μg/mL组穿过基质胶到达小室底端的细胞数目分别为对照组的(97.58±17.78)%、(67.95±49.75)%和(32.35±20.41)%。黄芩苷能够有效抑制HeLa细胞中MMP-2和MMP-9的表达及有效抑制HeLa细胞中P38的磷酸化水平；P38信号通路抑制剂预处理，能够增强黄芩苷对MMP-2和MMP-9表达的抑制作用。结论 黄芩苷能够有效抑制HeLa细胞的侵袭转移，这种作用可能与其通过调节P38信号通路的活性进一步调节MMP-2和MMP-9的表达有关。
ABSTRACT: Objective To observe the anti-metastatic effect and mechanism of baicalin on the growth of HeLa cells was measured by MTT assay, and cell migration baicalin on human cervical cancer HeLa cells. Methods The effects of baicalin on the proliferation and invasion of HeLa cells were analyzed by MTT method and Transwell assay. Moreover, Real-time PCR was used for investigating the expressions of MMP-2 and MMP-9 at the RNA level. Western blot was used for investigating the expressions of MMP-2, MMP-9, P38 and p-P38 at the protein level. Results Baicalin could significantly inhibit the proliferation of HeLa cells in the dose-dependent manner at the concentration above 60μg/mL. Anti-metastatic signaling induced by baicalin was characterized by down-regulating the RNA and protein expressions of MMP-2 and MMP-9, and down-regulating the phosphorylation level of P38. Pre-treatment of P38 signal pathway inhibitor could enhance the inhibitory effect of baicalin on the expressions of MMP-2 and MMP-9. Conclusion These results indicate that baicalin-induced anti-metastatic effect involves the inhibition of MMP-2 and MMP-9 in HeLa cells through P38 signal pathway