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- 2016
大气压低温等离子体处理BALB/c小鼠的安全性研究
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Abstract:
摘要：目的 使用大气压低温等离子(CAP)体处理BALB/c小鼠背部皮肤，验证CAP物理治疗对活体的安全性。方法 CAP每日照射小鼠背部皮肤3min，连续2周；检测处理组及对照组血常规及生化指标；测定小鼠脏器系数；对小鼠皮肤及脏器进行HE染色；测定小鼠精子活率及畸形率。结果 处理后小鼠背部皮肤及HE染色均未见异常；CAP处理组小鼠相比于对照组小鼠，在体质量、各脏器质量及各脏器系数均无统计学差异；CAP处理组小鼠的血常规指标及生化指标较对照组均未见明显改变，差异无统计学意义；两组小鼠的内脏大体外观形态正常，组间比较无明显差异；CAP处理组小鼠各内脏组织学观察较正常对照组未见明显异常改变；两组数据在统计学上无显著性差异。小鼠精子活率及畸形率两组数据在统计学上无显著性差异，CAP处理对雄性小鼠精子畸形无明显影响。结论 CAP处理对小鼠皮肤及各脏器无毒性作用，不产生生殖毒性，临床使用安全。
ABSTRACT: Objective To evaluate the biological safety of cold atmospheric plasma (CAP) treatment on the dorsal skin of BALB/c mice. Methods The dorsal skin of BALB/c mice was irradiated daily by three-minute CAP for two weeks. Both routine blood test and biochemical analysis were performed during this period. The indexes of internal organs were scored, followed by hematoxylin-eosin (HE) staining of these tissues and dorsal skin. The ratios of sperm activity and deformity were also calculated. Results After CAP treatment, the dorsal skin and H&E staining showed normal appearance. Compared with the controls, the mice in treatment group had the same weights and indexes of body and organs (P>0.05). Moreover, CAP did not affect the blood routine indexes or biochemical parameters (P>0.05). The two groups displayed similar optical and histological appearance of these internal organs. Furthermore, the two groups did not differ significantly in the ratios of sperm activity or deformity (P>0.05). Conclusion CAP treatment has clinical safety by exhibiting neither routine toxicity on the skin and internal organs nor genotoxicity on male reproductive system of BALB/c mice
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