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- 2016
骨髓间充质干细胞对大鼠心肌梗死后心肌营养素-1的影响
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Abstract:
摘要：目的 观察大鼠心肌梗死后心肌营养素-1(CT-1)的表达，并用骨髓间充质干细胞移植对其干预，探讨CT-1与心室重塑之间的关系。方法 随机选取Wistar雄性大鼠50只，10只作为假手术组(A组)；40只作为心肌梗死组，结扎左冠状动脉前降支(LAD)致心肌梗死24h后，分别随机抽取一半数量存活大鼠作为心肌梗死对照组(B组)及BMSC移植干预组(C组)。BMSC移植干预组(C组)通过尾静脉注射1mL BMSC细胞悬液于大鼠体内；A组、B组分别注射无血清培养液DMEM 1mL，6周后采用Real time PCR及免疫组化、Western blotting分别检测非梗死区CT-1的mRNA及蛋白表达量。结果 6周后，B组非梗死区心肌样本中，CT-1 mRNA及蛋白表达量、左心质量指数，同A、C组相比均显著升高，且差异有统计学意义(P<0.05)；C组非梗死区心肌中CT-1 mRNA及其蛋白含量、左心室质量指数均较A组显著升高，差异具有统计学意义(P<0.05)。与B组相比，C组以上指标则明显下降，差异有统计学意义(P<0.05)。结论 骨髓间充质干细胞能够抑制大鼠心肌梗死后CT-1 mRNA及蛋白表达，从而改善心肌重构。
ABSTRACT: Objective?? To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of cardiotrophin-1 (CT-1) in ventricular remodeling after acute myocardial infarction in rats. Methods?? We randomly divided 50 male Wistar rats into sham operation group A (sham operation group, n=10) and acute myocardial infarction group (n=40). Rats in acute myocardial infarction group were inflicted by ligating the left anterior descending coronary artery. After 24 hours, 40 survived rats were randomly divided into group B (myocardial infarction control group, n=20) and group C (BMSC group, n=20). Rats in group C were treated with BMSC transplantation, and those in group A and group B were treated with serum-free DMEM medium. After 6 weeks, the expressions of CT-1 at the mRNA and protein levels were detected by Real-time PCR method, immunohistochemical and Western blotting methods. Results?? ?After 6 weeks, the expressions of CT-1 at the mRNA and protein levels and left ventricular weight index in the myocardium of non-infraction zone in group B were significantly higher than those in group A and group C (P<0.05). The expressions of CT-1 mRNA and protein and left ventricular weight index in the myocardium of non-infraction zone in group C were significantly higher than those in group A (P<0.05). Conclusion?? BMSCs transplantation may improve ventricular remodeling after acute myocardial infraction in rats via inhibiting the expressions of CT-1 at the mRNA and protein levels
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