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- 2015
转化生长因子β1对牙周膜干细胞迁移、粘附及增殖的影响
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Abstract:
摘要：目的 研究转化生长因子β1(TGF-β1)对牙周膜干细胞(PDLSCs)的迁移、粘附和增殖的影响，初步探讨TGF-β1通过影响牙周组织干细胞参与牙周改建的机制。方法 分离培养人PDLSCs，鉴定其多向分化潜能。用Transwell法检测TGF-β1对PDLSCs的迁移的影响。用粘附实验检测TGF-β1对PDLSCs粘附能力的影响。用MTT法和生长率法检测TGF-β1对PDLSCs增殖的影响。 结果 浓度为2.5ng/mL以及10ng/mL的TGF-β1可促进PDLSCs的迁移，而且此作用呈剂量效应关系。粘附实验结果显示，在分别作用2h和4h后，浓度为10ng/mL的TGF-β1可促进PDLSCs的粘附。MTT法的结果证实了TGF-β1可促进PDLSCs的增殖，该作用呈剂量效应关系。再将细胞在含有或者不含有10ng/mLTGF-β1的培养基中培养2、4、6d，对细胞进行计数后发现TGF-β1显著促进PDLSCs的生长。结论 TGF-β1可促进PDLSCs的迁移、粘附和增殖。推测TGF-β1可能通过诱导PDLSCs向牙周组织部位迁移并提高其粘附和增殖能力。
ABSTRACT: Objective To evaluate the effects of transforming growth factor β1(TGF-β1) on migration, adhesion and proliferation of periodontal ligament stem cells (PDLSCs) and explore the mechanisms of PDLSCs-induced periodontal remodeling. Methods PDLSCs were isolated and identified from human teeth. The effect of TGF-β1 on migration of PDLSCs was evaluated using transwell migration assay. Cells attachment assay was used to test the effect of TGF-β1 on the adhesion of PDLSCs. In addition, the effect of TGF-β1 on the proliferation of PDLSCs was evaluated by MTT and cell growth rate assay. Results The results showed that TGF-β1 induced the migration of PDLSCs in a dose-dependent manner, improved the adhesion and proliferation of PDLSCs. So we propose that TGF-β1 may promote periodium remodeling by inducing PDLSCs migration, following adhesion and proliferation in these areas. Conclusion This study demonstrated for the first time that TGF-β1 increases the adhesion and migration of PDLSCs in vitro. The signal pathway is involved in the TGF-β1-induced migration of PDLSCs and the mechanical-chemical interaction during the orthodontic periodontal remodeling will be researched in our further studies
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