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- 2015
sCD14在痛风性关节炎患者炎症反应中的变化及其意义
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Abstract:
摘要：目的 探讨可溶性白细胞分化抗原14(sCD14)在痛风性关节炎(GA)患者炎症反应中的变化及其意义。方法 选取31例急性GA(AGA)患者、23例非AGA(NAGA)患者和20名健康对照者(HC)，用实时荧光定量聚合酶链反应(qRT-PCR)检测其外周血单个核细胞(PBMCs)中CD14的mRNA表达水平；用酶联免疫吸附法(ELISA)检测各组血浆sCD14、白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)蛋白表达水平，用免疫比浊法测定各组血浆C反应蛋白(CRP)含量；检测各组白细胞计数(WBC)、嗜中性粒细胞绝对值(GR)、淋巴细胞绝对值(LY)、单核细胞绝对值(MO)等血常规及血尿酸(UA)等指标变化，比较3组指标的差异并分析其与PBMCs的CD14 mRNA表达及血浆sCD14表达的相关性。结果 AGA组PBMCs中CD14 mRNA表达量高于HC组(P<0.05)；HC与NAGA组血浆sCD14蛋白表达低于AGA组(P<0.01)，且NAGA组表达低于HC组(P<0.05)；AGA组CRP蛋白水平高于HC与NAGA组(P<0.01)；AGA、NAGA组血浆IL-1β、TNF-α蛋白水平均显著高于HC组(P<0.01)，且AGA组IL-1β蛋白水平高于NAGA组(P<0.01)；AGA组和NAGA组WBC高于HC组(分别P<0.01，P<0.05)；AGA组GR、MO均高于HC组(P<0.05)，AGA组MO高于NAGA组(P<0.05)；AGA与NAGA组UA均显著高于HC组(P<0.01)。AGA组PBMCs的CD14 mRNA表达与IL-1？？SymbolbA@呈正相关(rs=0.362，P=0.045)；NAGA组血浆sCD14蛋白表达与GR呈正相关(rs=0.397，P=0.030)。结论 sCD14在GA患者的炎症反应中表达异常，提示sCD14在GA患者的炎症反应中可能发挥重要的调控作用。
ABSTRACT: Objective To study the changes and significance of sCD14 in inflammatory response of patients with gouty arthritis. Methods CD14 mRNA was measured using quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs). The expression of CD14 mRNA in PBMCs was compared between patients with acute gouty arthritis (AGA) (n=31) and non-acute gouty arthritis (NAGA) (n=23) and healthy controls (HC) (n=20). β-actin was selected as the internal control. The protein expressions of sCD14, IL-1β and TNF-α were measured using enzyme linked immunosorbent assay (ELISA) in patients’ plasma. The protein expression of CRP was measured using immunoturbidimetry in patients’ plasma. Routine blood and blood biochemistry indexes were measured by routine blood analyzer and blood biochemistry analyzer of patients with AGA, NAGA and HC. We analyzed the correlation between CD14 mRNA, sCD14 protein expression and each clinical indicator. Results When compared with that in AGA group, the mRNA expression of CD14 increased significantly in PBMCs of HC patients (P<0.05). When compared with that in HC and NAGA patients, the protein expression of sCD14 increased significantly in the plasma of AGA patients (P<0.01). The protein expression of sCD14 was significantly lower in the plasma of NAGA than in HC (P<0.05). The protein expression of sCD14 increased significantly in the plasma of AGA compared with HC and NAGA (P<0.01). When compared with those in HC, the protein expressions of IL-1β and TNF-α increased significantly in the plasma of AGA and NAGA (P<0.01). When compared with that in NAGA, the protein expression of IL-1β increased significantly in plasma of AGA (P<0.01). The indexes of WBC
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