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- 2015
多发性骨髓瘤小鼠注射重组鼠源促红细胞生成素后对免疫功能的影响
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Abstract:
摘要：目的 探讨重组人红细胞生成素(rhEPO)对多发性骨髓瘤小鼠免疫功能的影响。方法 建立多发性骨髓瘤小鼠模型，随机分为4组，即对照组和高、中、低剂量治疗组，治疗组分别给予尾静脉注射重组人源促红细胞生成素2.5、5、10mg/kg，容量为100μL，连续给药30d，对照组给予尾静脉注射等体积生理盐水。实验结束后，采用MTT法测定各组小鼠T、B淋巴细胞增殖能力，中性红实验测定巨噬细胞吞噬能力和ELISA试剂盒测定小鼠血清中TNF-α含量。结果 与对照组相比，不同剂量rhEPO治疗组小鼠B、T淋巴细胞增殖能力、巨噬细胞吞噬能力和血清中TNF-α含量均显著提高(P<0.05)。结论 重组人红细胞生成素可显著改善多发性骨髓瘤小鼠免疫调节能力。
ABSTRACT: Objective To investigate the effect of recombinant human erythropoietin on immune function in mice with multiple myeloma. Methods Multiple myeloma model mice were divided randomly into control group and treatment groups (low, middle and high doses). The rats in treatment groups were injected with recombinant human erythropoietin 100μL (2.5, 5.0 and 10mg/kg) via the tail vein for 30 days, and the rats in control group were injected isotonic saline solution of the same volume instead. After the experiment, the colorimetric MTT assay, neutral red method and ELISA kit were used to evaluate the proliferation of B and T lymphocytes, the phagocytic ability of macrophages and the content of serum TNF-α. Results Compared with those in control group, different doses of recombinant human erythropoietin could significantly increase the proliferation of lymphocytes, increase the phagocytosis of macrophages and improve the production of serum TNF-α(P<0.05). Conclusion Recombinant human erythropoietin can significantly improve immunoregulation of mice with multiple myeloma
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