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- 2015
小鼠骨关节炎模型中胫骨软骨下骨微结构的Micro-CT分析
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Abstract:
摘要：目的 应用显微CT(Micro-CT)技术观察和分析小鼠骨关节炎模型中软骨下骨微结构的变化，为进一步了解软骨下骨在骨关节炎疾病发展中的作用提供一定的实验基础。方法 野生型小鼠12只，随机均分为内侧半月板-胫骨韧带切断术组(DMM组)和对照组(假手术组)。术后8周，取两组动物术侧全膝关节置于Micro-CT仪器中进行扫描，应用三维重建处理和分析软件对内侧胫骨平台软骨下骨全部及其外、中、内1/3三个亚区域的骨体积分数(BV/TV)、骨小梁厚度(Tb.Th)、骨小梁数量(Tb. N)、骨小梁分离度(Tb.Sp)和组织骨密度(TMD)进行检测，并行膝关节的病理组织学染色。 结果 与对照组比较，DMM实验组小鼠在Micro-CT成像和组织学染色上均出现软骨下骨骨小梁增宽、致密、排列紊乱不均匀等表现，关节内侧缘可见骨赘形成；在全部亚区域的Tb.Th、外1/3亚区域的BV/TV与TMD、中1/3亚区域的BV/TV、Tb.Th与TMD、内1/3亚区域的Tb.Th均升高，而在全部亚区域的Tb.N、外1/3亚区域的Tb.Sp、中1/3亚区域的Tb.N与Tb.Sp、内1/3亚区域的BV/TV与Tb.N均降低。结论 对小鼠DMM骨关节炎模型的胫骨软骨下骨多个亚区域进行微结构的Micro-CT测量分析可获得小鼠模型更加全面的数据资料。
ABSTRACT: Objective To analyze the subchondral-bone microstructure in a mouse model of osteoarthritis (OA) via micro-CT technology so as to provide experimental basis for further study of subchondral bone during the development of osteoarthritis. Methods Twelve wild-type mice were randomly divided into DMM (destabilization of the medial meniscus) group and control (sham operation) group. All the knee joints for surgery from the two groups were scanned by micro-CT equipment, reconstructed and analyzed to acquire data of bone volume fraction (BV/TV), trabecular thickness (Tb. Th), trabecular number (Tb.N), trabecular separation (Tb.Sp) and tissue mineral density (TMD) from the total, outer, middle, and inner 1/3 compartments of subchondral bone in medial tibial plateau 8 weeks after surgery. Histological staining was also applied. Results Compared with those in control group, extended, denser and uneven trabeculae and osteophyte formation from micro-CT imaging and histology were observed in the subchondral bone of DMM-induced mice. There were higher Tb. Th in total, BV/TV and TMD in outer 1/3, BV/TV, Tb. Th and TMD in middle 1/3, and Tb. Th in inner 1/3 compartments, while there were correspondingly lower Tb. N in total, Tb. Sp in outer 1/3, Tb. N and Tb. Sp in middle 1/3, BV/TV and Tb. N in inner 1/3 compartments. Conclusion More comprehensive data could be acquired from the microstructure of subchondral-bone compartments by micro-CT analysis in the mouse model of DMM-induced OA
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