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- 2017
siRNA沉默EZH2基因对人宫颈癌细胞侵袭转移的影响
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Abstract:
摘要：目的 探讨靶向沉默EZH2基因对宫颈癌细胞侵袭转移的影响及其分子机制。方法 采用化学合成siRNA瞬时转染人宫颈癌C33A细胞，沉默EZH2基因的表达。通过细胞划痕实验、Transwell小室侵袭实验、软琼脂克隆形成实验，观察沉默EZH2对宫颈癌细胞迁移、侵袭及体外成瘤能力的影响，Western blot法检测MMP2的表达变化。结果 EZH2 siRNA转染C33A细胞后，细胞划痕及Transwell小室侵袭实验结果均显示宫颈癌细胞的迁移、侵袭能力明显减低，差异具有统计学意义；软琼脂克隆形成实验表明，EZH2沉默后克隆形成数目明显减少，差异具有统计学意义；Western blot结果显示MMP2表达水平下调。结论 沉默EZH2基因表达能显著抑制宫颈癌C33A细胞的侵袭转移能力，其作用机制可能通过下调MMP2来实现。
ABSTRACT: Objective To investigate the effects of siRNA silencing enhancer of zeste homolo 2 (EZH2) on invasion and migration of cervical cancer cells and the molecular mechanisms. Methods The small interfering RNAs (siRNA) targeting EZH2 were transiently transfected into C33A cell line by lipofectamin2000. The effects of EZH2 on cell invasion and migration were detected by wound-healing assay, Transwell assay and soft agar colony assay. The expression of MMP2 was detected by Western blot. Results Downregulation of EZH2 expression by siRNA in C33A cell line significantly inhibited cell invasion and migration in vitro. Meanwhile, siRNA-mediated depletion of EZH2 reduced the expression of MMP2. Conclusion Knocking down EZH2 expression by siRNA could surpress invasion and migration of human cervical cancer cells, which might be related to downregulating MMP2 expression
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