右美托咪定对离体大鼠肠系膜动脉炎性相关蛋白表达的影响
Effects of dexmedetomidine on expressions of mesenteric artery proteins in rats

摘要：目的 研究右美托咪定(DEX)对大鼠肠系膜动脉肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)及凋亡相关因子Caspase-3表达？A作用。方法 取SPF级SD雄性大鼠，脱臼处死后，在体视显微镜下分离取出肠系膜动脉。建立脂多糖(LPS)诱导的血管损伤实验模型，并根据随机化原则将损伤血管分为DEX组和对照组。DEX组根据所给予DEX的浓度不同分为10-8、10-7、 10-6mol/L 3个亚组。分离提取各组动脉组织中RNA及总蛋白后，RT-PCR方法检测TNF-α、IL-1β及CaSR等因子的mRNA水平；Western blot检测TNF-α、CaSR、AMPK及Caspase-3等蛋白的表达变化。 结果 10-8、10-7、10-6mol/L DEX组中 LPS诱导的血管炎性反应均明显降低，TNF-α的mRNA和蛋白表达及IL-1β的mRNA表达减弱。DEX组血管条的Caspase-3蛋白表达较LPS组显著降低。DEX对LPS诱导的血管AMPK蛋白表达和CaSR的mRNA表达没有明显作用。结论 DEX明显降低与炎症相关蛋白的表达，提示DEX有一定的抗炎症作用。
ABSTRACT: Objective To observe the effects of dexmedetomidine (DEX) on the expressions of TNF-α, IL-1β and apoptosis-related proteins in rat mesenteric artery. Methods Male SD rats of SPF grade were sacrificed and the mesenteric artery was separated under the stereo-microscope. We established an experimental model of vascular injury induced by lipopolysaccharide (LPS) and randomly divided the injured vessels into dexmedetomidine treatment group and control group. DEX treatment group was divided into 10-8, 10-7, and 10-6mol/L subgroups according to the different concentrations of DEX. RNA and total protein was extracted in each group. The mRNA expressions of TNF-α, IL-1β and CaSR were detected by RT-PCR and the protein expression of TNF-α, Caspase-3 and AMPK were tested by Western blot. Results DEX (10-8, 10-7, and 10-6mol/L) obviously reduced vascular inflammatory reaction induced by lipopolysaccharide, reduced the mRNA and protein expressions of TNF-α as well as mRNA expression of IL-1β. Caspase 3 protein expression significantly lowered in blood vessels in DEX group compared with LPS group. DEX had no obvious effect on lipopolysaccharide-induced vascular AMPK and CaSR mRNA or protein expressions. Conclusion DEX obviously deceased the expressions of inflammation-related proteins, suggesting that DEX has anti-inflammatory effects