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- 2017
丁基苯酞对痴呆模型大鼠认知能力及线粒体功能的作用
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Abstract:
摘要：目的 观察丁基苯酞对Aβ25-35致痴呆大鼠学习记忆的改善情况，并初步探索其作用机制。方法?? 实验设立正常组、模型组和丁基苯酞组(10、30、100mg/kg)，模型组和丁基苯酞组均采用侧脑室注射Aβ25-35，干预组造模后给予丁基苯酞灌胃(灌胃剂量为2.5mL/kg)。利用水迷宫实验观察大鼠的学习记忆能力，采用ATP检测观察脑组织内线粒体功能的变化，并用试剂盒检测线粒体相关酶活性的变化。结果 水迷宫实验结果显示，模型组大鼠学习记忆能力与正常组比较差异有统计学意义(P<0.05)，丁基苯酞10mg/kg组、30mg/kg组大鼠学习记忆障碍均有明显改善(P<0.05)，100mg/kg组改变不明显；线粒体相关检测发现，与正常组相比，模型组ATP水平明显下降(P<0.05)，线粒体呼吸链复合物Ⅳ(P<0.05)、丙酮酸脱氢酶复合体、α-酮戊二酸脱氢酶活性均明显降低(P<0.05)。与模型组相比，丁基苯酞能明显提高线粒体相关酶的活性从而改善线粒体功能。结论 丁基苯酞可以通过改善线粒体功能，提高Aβ25-35致痴呆大鼠的学习记忆能力。
ABSTRACT: Objective??? To explore the protective effects of butylphthalide against?Aβ25-35 induced dementia-like pathological rat model and reveal the mechanism. Methods? ?Rats were divided into five groups: control group, model group and NBP groups (10, 30, and 100mg/kg). In model group and butylphthalide groups,?Aβ25-35 was injected into the lateral ventricle, while the rats in intervention group were administered with butylphthalide (gastric infusion dose of 2.5mL/kg). Learning and memory abilities of the rats were observed with water maze test. Mitochondrial function in brain tissue was observed by ATP assay, and the mitochondrial related enzyme activities were detected by the kit. Results??? Water maze test showed that learning and memory abilities of model group were poorer than those of control group. They were significantly improved in NBP 10mg/kg group and 30mg/kg group (P<0.05), but did not change significantly in 100mg/kg group. Compared with control group, model group had significantly decreased ATP level (P<0.05); cytochrome c oxidase, pyruvate dehydrogenase complex and ketoglutarate dehydrogenase activities were also significantly decreased (P<0.05). Compared with model group, butylphthalide group had significantly improved activities of mitochondrial enzymes that improved mitochondrial function. Conclusion?? Butylphthalide can improve learning and memory abilities of rats with Aβ25-35-induced dementia by improving mitochondrial function
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