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- 2015
大蒜素对脑胶质瘤细胞U87细胞侵袭能力的影响及其机制
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Abstract:
摘要：目的 检测大蒜素对脑胶质瘤细胞U87侵袭能力的影响及其可能机制。方法 利用MTT法检测大蒜素对U87细胞增殖能力的抑制作用。利用Transwell小室检测大蒜素对U87细胞侵袭能力的抑制作用。采用Real-time PCR和Western blotting方法检测大蒜素对U87细胞中MMP-2和MMP-9表达水平的影响。采用Western blotting 的方法检测大蒜素对p38磷酸化水平的影响。结果 大蒜素能够明显抑制U87细胞的增殖(浓度>8μg/mL，P<0.05)。且大蒜素(浓度<8μg/mL)能够明显抑制U87细胞的侵袭能力(P<0.05)。在大蒜素作用24h后，U87细胞中MMP-2和MMP-9的表达水平明显降低(P<0.05)。p38的磷酸化水平在大蒜素作用后明显降低(P<0.05)。结论 大蒜素能够抑制脑胶质瘤细胞U87的侵袭能力，其机制可能是通过对p38信号的通路活性的调节进而降低MMP-2和MMP-9的表达来实现的，提示大蒜素在脑胶质瘤的治疗中具有潜在的应用价值。
ABSTRACT: Objective To investigate the anti-metastatic effect of allicin on glioma cell line U87 and related mechanisms. Methods In this study, we employed MTT assay to test the anti-proliferative effect of allicin. Transwell assay was used to test the anti-metastatic ability of allicin. Real-time PCR and Western blotting were employed to test the effect of allicin on the expressions of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9). Western blotting was employed to test the phosphorylated level of p38. Results Allicin could significantly inhibit the proliferation and invasion of U87 cells (concentration>8μg/mL, P<0.05). Meanwhile allicin (concentration<8μg/mL) could inhibit the invasion of U87 cells. After treatment with allicin for 24 hours, the expressions of MMP-2 and MMP-9 were decreased significantly (P<0.05). Moreover, allicin treatment decreased the phosphorylated level of p38 obviously (P<0.05). Conclusion Allicin inhibits the invasion and migration of glioma cell line U87 by reducing the expressions of MMP-2 and MMP-9 via suppressing the activity of p38 signal pathway, suggesting that allicin is a potential therapeutic agent for glioma
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