Ad36感染对维吾尔族肥胖患者progranulin表达的调节作用
The regulation of adenovirus type 36 infection and progranulin expression in Uygur obese patients

摘要：目的 探讨腺病毒36型(Ad36)感染对维吾尔族肥胖患者颗粒蛋白前体(progranulin)表达的调节作用。方法 根据肥胖诊断标准将维吾尔族人群样本分为肥胖组(115)与非肥胖组(117)，收集血清标本232份，脂肪标本102份。血清中和反应法检测样本血清中的Ad36抗体。采用实时荧光定量PCR(real time quantitative-PCR)方法检测研究对象大网膜和皮下的脂肪组织progranulin的mRNA表达水平，利用酶联免疫吸附实验(ELISA)测定血清progranulin的蛋白表达水平。免疫组化方法检测脂肪组织巨噬细胞CD68蛋白表达，了解巨噬细胞浸润情况。结果 ①肥胖患者Ad36感染率(54/115,47.0%)明显高于非肥胖者(38/117,32.5%)，差异有统计学意义(P<0.05)。②Ad36感染的肥胖组血清progranulin的蛋白表达水平(408.45±156.92)显著高于非肥胖组(326.11±158.60)，差异有统计学意义(P<0.05)；两组的腹部大网膜、皮下脂肪组织progranulin mRNA表达水平差异无统计学意义(P均>0.05)。③Ad36感染的肥胖组巨噬细胞浸润(14730.16±2227.39)明显高于非肥胖组(10786.50±2772.80)，差异有统计学意义(P<0.05)。 结论 Ad36感染可能与维吾尔族肥胖发生有关，其机制可能为通过调节血清progranulin的蛋白表达水平而实现。
ABSTRACT: Objective To investigate the regulation of adenovirus type 36 infection on precursor protein particles progranulin expression in Uygur obese patients. Methods Based on the diagnostic criteria of obesity, the samples were divided into obese group and non-obese group. Serum neutralization test was used to detect the antibody of Ad36. The progranulin mRNA expressions in abdominal omental and subcutaneous adipose tissues were detected by real-time quantitative PCR. ELISA method was used to determine serum progranulin protein levels. CD68 protein expression of macrophage was detected by immunohistochemistry. Results ① In the Uygur population of our study, compared with that in the non-obese group (38/117, 32.5%), the number of obese patients (54/115, 47.0%) infected with Ad36 was significantly higher than that in non-obese group (P<0.05). ② Serum progranulin was significantly increased in the Ad36-infected obese group (408.45±156.92) than in non-obese group (326.11±158.60) (P<0.05). The mRNA expression of progranulin did not differ between the two groups. ③ The macrophage infiltration was significantly higher in the Ad36-infected obese group (14730.16±2227.39) than in non-obese group (10786.50±2772.80) (P<0.05). Conclusion Ad36 infection may be associated with the occurrence of obesity in Uygur population, and adenovirus type 36 infection may regulate the expression of serum progranulin at the protein level