miR-125a-3p和miR-17-5p在激素性股骨头坏死中的作用
Effects of miR-125a-3p and miR-17-5p on steroid-induced osteonecrosis

摘要：目的 探讨激素性股骨头坏死中miRNA的差异性表达变化。方法 取激素性股骨头坏死的坏死区域骨组织为实验组，其股骨颈基底部骨组织为对照组；分别提取总RNA并进行质检；采用miRNA芯片技术检测miRNAs的表达并对其表达谱进行分析；运用实时定量PCR对差异明显的miR-125a-3p和miR-17-5p进行验证。结果 对激素性股骨头坏死中miRNA表达谱进行差异性分析。和对照组相比，实验组中倍数大于2倍，P<0.05的miRNA共有11个。8个miRNA表达上调，3个表达下调。实时定量PCR证实miR-125a-3p在坏死标本中高表达，而miR-17-5p呈现低表达，与miRNA芯片结果一致。结论 激素性股骨头坏死区域骨组织与对照组相比miRNAs表达发生明显变化，以miR-125a-3p和miR-17-5p差异最为明显。这表明了它们最有可能参与了激素性股骨头坏死病理过程的调控。
ABSTRACT: Objective To explore the differential expression of microRNAs (miRNAs) in steroid-induced osteonecrosis (ON). Methods Bone tissues were selected from patients with steroid-induced ON between the necrotic zone of femoral heads and its femoral neck to analyze the miRNA expression profile using the microarray. The most differentially expressed miR-125a-3p and miR-17-5p in microarray analysis were further confirmed by real-time quantitative PCR. Results According to the microarray screening, 8 miRNAs were upregulated and 3 miRNAs were downregulated in the necrotic zone of femoral heads samples (Fold>2, P<0.05). Results of real-time PCR revealed that miR-125a-3p was upregulated and miR-17-5p was downregulated in the necrotic zone of femoral heads samples, which were in agreement with the microarray data. Conclusion MiRNA’s differential expression profile of human steroid-induced ON samples was obtained. Among the differentially expressed miRNAs, miR-125a-3p and miR-17-5p are the most apparently differentially expressed miRNAs, which may be related to the pathogenesis and development of steroid-induced ON