趋化因子受体CX3CR1在大鼠重症急性胰腺炎中的表达和意义
Expression and significance of chemokine receptor CX3CR1 in rats with severe acute pancreatitis

摘要：目的 探讨趋化因子受体CX3CR1在重症急性胰腺炎(severe acute pancreatitis, SAP)大鼠模型中的表达，判断其能否作为SAP发生的预测指标。方法 40只Sprague-Dawley大鼠随机分为假手术组(sham operation, SO)(n=20)和SAP模型组(n=20)，分别于制模后6、12、24、48h处死大鼠，应用酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)法检测SAP大鼠血清中CX3CR1的变化，同时应用免疫组织化学法检测SAP大鼠胰腺、肺脏及肾脏组织中CX3CR1蛋白的表达。结果 大鼠SAP制模成功后，血清中CX3CR1的表达逐渐升高，24h达最高(542.4pg/mL)，明显高于SO组(P<0.05)。大鼠SAP组的胰腺、肺脏和肾脏组织中均有CX3CR1的表达，且明显高于SO组，其中胰腺和肺脏组织中CX3CR1表达在制模24h达高峰，肾脏组织中CX3CR1表达在制模48h达高峰。结论 趋化因子受体CX3CR1可能为预测SAP严重程度的有效指标之一，值得进一步深入研究。
ABSTRACT: Objective To explore the expression of chemokine receptor CX3CR1 in severe acute pancreatitis (SAP) rats model and to estimate whether it can be a potential predictor of SAP. Methods Forty Sprague-Dawley rats were randomly divided into sham-operation (SO) group (n=20) and SAP group (n=20). Rats were killed at 6h, 12h, 24h and 48h after model induction. The serum level of CX3CR1 was measured by enzyme-linked immunosorbent assay (ELISA). The expression of CX3CR1 protein in the pancreas, lung and kidney were detected by immunohistochemistry. Results The serum level of CX3CR1 in SAP rats increased gradually after model induction and reached the peak at 24h (542.4pg/mL), which were significantly higher than those in SO group (P<0.05). The expression of CX3CR1 was found in the pancreas, lung and kidney tissues of SAP rats and higher than that in SO group. Meanwhile, the expression of CX3CR1 reached the peak at 24h in the pancreas and lung and at 48h in the kidney. Conclusion This study suggests that the chemokine receptor CX3CR1 may be one effective index for predicting the severity of acute pancreatitis and deserves further research