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- 2016
microRNA-491-5p血浆水平及其靶基因多态性与早发冠心病易感性的研究
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Abstract:
摘要：目的 通过病例对照研究，探讨陕西省汉族人群血浆miRNA-491-5p水平和miRNA-491-5p(has-miR-491-5p)靶基因基质金属蛋白酶-9(MMP-9)单核苷酸多态性(SNP)改变与早发冠心病(pCAD)发生风险及预后的关系。方法 连续收集pCAD病例270例，对照组300例。用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP)检测has-miR-491-5p靶基因MMP-9 rs1056628的多态性，同时比较各种基因型与pCAD发生的相关性。结果 rs1056628位点存在A-C多态性，与CC基因型(发生率42%)相比，携带CA、AA基因型的个体冠心病的发生率为31%，差异有统计学意义(P=0.045)；携带CA/AA基因型冠心病在低水平总胆固醇(TC)、较低水平的低密度脂蛋白胆固醇(LDL-C)人群中的患病风险降低更为显著。结论 has-miRNA-491-5p靶基因MMP-9 rs1056628C-A多态性改变可降低pCAD的发生率，携带C等位基因是pCAD发病的独立危险因素。
ABSTRACT: Objective To study the relationship of the plasma levels of miRNA-491-5p in Han population in Shaanxi Province and the changes of single nucleotide polymorphisms (SNP) of the target gene matrix metalloproteinases-9 (MMP-9) of miRNA-491-5p (has-miR-491-5p) with the incidence risk and prognosis of premature coronary artery disease (pCAD) through the case-control design. Methods In this study, we made a consecutive recruitment of 270 pCAD cases in the case group and 300 cases in the control group. Using the polymorphism method of polymerase chain reaction and restriction fragment length (PCR-RFLP), target gene MMP-9 of has-miR-491-5p and rs1056628 genotypes was detected to compare the association between the variant genotypes and pCAD. Results In the changes of rs1056628C-A polymorphisms, compared with that of CC genotypes (the incidence was 42%), the risks of having coronary heart disease in the individuals carrying CA and AA genotypes were 31%, the difference was statistically significant (P=0.045). The risks of developing coronary heart disease in the individuals carrying CA and AA genotypes were reduced more significantly in the population with low total cholesterol (TC), and low low-density lipoprotein cholesterol (LDL-C). Conclusion Target gene MMP-9 of has-miRNA-491-5p rs1056628C-A polymorphism is associated with the reduced incidence risk of pCAD, and carrying C alleles is an independent risk factor for pCAD
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