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- 2017
Clusterin、E-cadherin在宫颈鳞癌组织中的表达及其相关性分析
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Abstract:
摘要：目的 探讨Clusterin和E-cadherin在宫颈鳞癌组织中的表达及其相关性。方法 采用免疫组织化学染色法，检测18例正常宫颈组织、32例宫颈上皮内瘤变(CIN)组织以及40例宫颈鳞状细胞癌(CSC)组织中Clusterin和E-cadherin的表达。结果 3组患者的基线资料差异无统计学意义。宫颈鳞癌、CIN2、CIN3中Clusterin的表达均高于正常宫颈组织(P<0.05)。Clusterin在Ⅰ级宫颈鳞癌中的表达低于Ⅲ级(P<0.01)，并与临床分期有关(P<0.05)，而与淋巴结转移、癌灶浸润间质深度无关(P>0.05)。E-cadherin在宫颈鳞癌及CIN2、CIN3中的表达均低于正常宫颈组织(P<0.05)。E-cadherin在Ⅰ级、Ⅱ级宫颈鳞癌中的表达均高于Ⅲ级(P<0.05)，并与淋巴结转移有关(P<0.05)，而与临床分期、癌灶浸润间质深度无关(P>0.05)。宫颈鳞癌组织中Clusterin和E-cadherin的表达呈负相关(r=－0.339，P<0.05)。结论 Clusterin的高表达与E-cadherin的低表达可能与宫颈鳞癌的发生有关，联合检测二者的表达可能对宫颈鳞癌的早期诊断具有潜在的临床价值。
ABSTRACT: Objective To explore the expressions of clusterin and E-cadherin in cervical squamous tissues and their correlation. Methods Immunohistochemical technique was used to measure the expressions of clusterin and E-cadherin in 18 normal cervical tissues, 32 cervical intraepithelial neoplasia (CIN) tissues and 40 cervical squamous carcinoma (CSC) tissues. Results There were on significant differences in baseline characteristics among the three groups based on standardized differences. The expressions of clusterin in CSC, CIN2 and CIN3 tissues were significantly higher than those in normal cervical tissues (P<0.05). The expression of clusterin was significantly lower in grade Ⅰ than in grade Ⅲ (P<0.01) and was connected with clinical stage (P<0.05) instead of lymph node metastasis and cancer stromal invasion depth (P>0.05). The expressions of E-cadherin in tissues of CSC, CIN2 and CIN3 were significantly lower than those in normal cervical tissues (P<0.05). E-cadherin expression was both significantly higher in grade Ⅰ and grade Ⅱ than in grade Ⅲ (P<0.05), and was associated with lymph node metastasis (P<0.05) rather than clinical stage and cancer stromal invasion depth (P>0.05). A negative correlation was found between the expressions of clusterin and E-cadherin in CSC (r=－0.339, P<0.05). Conclusion The significantly different expressions of clusterin and E-cadherin may be related to the development of CSC, suggesting that the two may be used as potential markers for early diagnosis of CSC
| [1] | MAJUMDAR AP, DU J, HATFIELD JS, et al. Expression of EGF-receptor related protein (ERRP) decreases in gastric mucosa during aging and carcinogenesis[J]. Dig Dis and Sci, 2003, 48(5):856-864. |
| [2] | MOON HS, PARK WI, CHOI EA, et al. The expression and tyrosinephosphorylation of E-cadherin/catenin adhesion complex, and focal adhesion kinase in invasive cervical carcinomas[J]. Int J Gynecol Cancer, 2003, 13(5):640-646 |
| [3] | SINICROPE FA, RUAN SB, CLEARY KR, et al. bcl-2 and p53 Oncoprotein expression during colorectal tumorigenesis[J]. Cancer Res, 1995, 55(2):237-241. |
| [4] | LEE JY, KIM HJ, RHO SB, et al. eIF3f reduces tumor growth by directly interrupting clusterin with anti-apoptotic property in cancer cells[J]. Oncotarget, 2016, 14(7):18541-18557. |
| [5] | CHEN D, WANG Y, ZHANG K, et al. Antisense oligonucleotide against clusterin regulates human hepatocellular carcinoma invasion through transcriptional regulation of matrix metalloproteinase-2 and e-cadherin[J]. Int J Mol Sci, 2012，13(8):10594-10607. |
| [6] | LINDSEY AT, FREDDIE B, REBECCA LS, et al, Global cancer statistics, 2012[J]. CA Cancer J Clin, 2015, 65(2):87-108. |
| [7] | WANG Y, LIU YH, MAI SJ, et al. Evaluation of serum clusterin as a surveillance tool for human hepatocellular carcinoma with hepatitis B virus related cirrhosis[J]. J Gastroenterol Hepatol, 2010, 25(6):1123-1128. |
| [8] | YANG GF, LI XM, XIE D. Overexpression of clusterin in ovarian cancer is correlated with impaired survival[J]. Int J Gynecol Cancer, 2009, 19(8):1342-1346. |
| [9] | SUN B, ZHANG S, ZHANG D, et al. Clusterin is associated with spontaneous breast cancer in TA2 mice[J]. FEBs Lett, 2007, 581(17):3277-3282. |
| [10] | REDONDO M, RODRIGO I, ALCAIDE J, et al. Clusterin expression is associated with decreased disease-free survival of patients with colorectal carcinomas [J]. Histopatholory, 2010, 25(6):1123-1128. |
| [11] | GLOUSHANKOVA NA. Changes in regulation of cell-cell adhesion during tumor transformation[J]. Biochemistry(Mosc), 2008, 73(7):742-750. |
| [12] | KLOKOV D, CRISWELL T, IESKOV KS, et a1. IR-inducible clusterin gene expression: A protein with potential roles in ionizing radiation induced adaptive responses, genomic instability, and bystander effects[J]. Mutat Res, 2004, 568(1):97-110. |
| [13] | KOAY MH, CROOK M, STEWART CJ. Cyclin D1, E-cadherin and beta-catenin expression in FIGO IA cervical squamous carcinoma: Diagnostic value and evidence for epithelial-mesenchymal transition [J]. Histopathology, 2012, 61(6):1125-1133. |
| [14] | GRILLE SJ, BELLACOSA A, UPSON J, et al. The protein kinase Akt induces epimelial mesenchymal transition and promotes enhanced motility and invasiveness of squamous cell carcinoma lines[J]. Cancer Res, 2003, 63(9):2172-2178. |
| [15] | YAN Y, LUO K, ZHANG H, et al. RNA interference-mediated secretory clusterin gene silencing inhibits proliferation and promotes apoptosis of human non-small cell lung cancer cells[J]. Hepatogastroenterology, 2013, 60(121):70-75. |
