六磷酸肌醇对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响
Effects of inositol hexaphosphate on proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts

摘要：目的 研究六磷酸肌醇(inositol hexaphosphate, IP6)对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响，并探求其抗癌效果的最佳作用浓度。方法 原代培养人成骨细胞并鉴定，复苏并常规培养人骨肉瘤细胞株？？MG-63？？，MTT法检测不同浓度IP？？6干预培养时两种细胞的增殖情况，确定IP6的最佳抑癌浓度，并进一步用流式细胞仪检测MG-63细胞周期及凋亡情况。结果 当IP6浓度≥1mmol/L时开始抑制MG-63细胞增殖，在4mmol/L时达到最大，并呈时间-剂量依赖性；当IP6浓度为0.5、1mmol/L时，对成骨细胞增殖抑制不明显，2mmol/L时轻度抑制，4mmol/L时导致成骨细胞凋亡。结论 IP6能够抑制人骨肉瘤细胞MG-63的增殖并导致其凋亡，其抗癌的最佳作用浓度为2mmol/L。
ABSTRACT: Objective To investigate the effects of inositol hexaphosphate (IP6) on proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts so as to explore the optimal concentration for achieving anti-cancer effects. Methods We primary cultured and identified human osteoblasts. Then we made recovery and normal culture of human osteosarcoma cell line MG-63. We tested the proliferation of two kinds of cell lines under different concentrations of IP6 by MTT to determine the optimal concentration and then detected MG-63 cell cycle and apoptosis by flow cytometry. Results When IP6 concentration was more than 1mmol/L, IP6 began to inhibit the proliferation of MG-63 cell line in the time-dose dependent manner. When the concentration reached 4mmol/L, this inhibitory effect was the maximum. When IP6 concentration was 0.5mmol/L or 1mmol/L, the proliferation of osteoblasts was not obviously inhibited. When it was 2 mmol/L, the proliferation was slightly inhibited. A concentration of 4mmol/L caused the apoptosis of osteoblasts. Conclusion IP6 can inhibit the proliferation of osteosarcoma cell line MG-63 and lead to its apoptosis. The optimal concentration is 2mmol/L for achieving anti-cancer effects