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- 2016
沉默IFITM1基因对卵巢癌CP70细胞增殖和侵袭能力的影响
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Abstract:
摘要：目的 探讨siRNA沉默IFITM1基因表达对卵巢癌CP70细胞增殖和侵袭的影响。方法 利用脂质体转染法将靶向IFITM1的小干扰RNA(siRNA)转染卵巢癌细胞株CP70；qRT-PCR和Western blot观察转染后CP70细胞IFITM1 mRNA和蛋白表达的变化；平板克隆形成实验和Transwell小室分别检测各组细胞增殖和侵袭能力的变化。结果 转染组CP70细胞的IFITM1 mRNA和蛋白表达明显受抑制；转染组、阴性对照组及转染试剂对照组形成克隆数分别为84、181、178，克隆形成率分别为42%、90.5%、89%，迁移出的细胞分别约为59个、121个、126个，转染组结果与其他两组之间有统计学差异，说明转染IFITM1siRNA抑制了卵巢癌CP70细胞增殖和侵袭能力。结论 沉默IFITM1可以降低卵巢癌细胞的增殖和侵袭能力，IFITM1可能成为卵巢癌治疗的潜在新靶点。
ABSTRACT: Objective To investigate the inhibitory effect of synthetic interferon-induced transmembrane protein 1(IFITM1) siRNA on the proliferation and migration of human ovarian cancer cell line CP70. Methods The siRNA targeted IFITM1 was transfected into CP70 cells by LipofectamineTM 2000. Expressions of IFITM1 mRNA and protein were examined by qRT-PCR and Western blot. Plate clone assay and Transwell chamber were used to observe the proliferation and migration of CP70 cells. Results IFITM1 siRNA significantly inhibited the expression of IFITM1 in human ovarian cancer cell line CP70 at both mRNA and protein levels. The colony formation assay indicated that the clone number was 84 in IFITM1siRNA, which was much fewer than 181 in negative control group and 178 in mock transfection group. The colony-forming efficiency (CFE) was 42%, 90.5% and 89%, respectively. Transwell chamber results showed that the number of migrated cells was 59, 121 and 126, respectively; the siRNA transfection group differed significantly from the other two groups, indicating that downregulated IFITM1 expression greatly inhibited the proliferation and migration of CP70 cells. Conclusion Knockdown of IFITM1 inhibited the proliferation and migration of CP70 cells. IFITM1 is a potential therapeutic target for human ovarian cancer
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