TRX1与JAB1在急性髓系白血病中的表达及其相关性
Relationship between TRX1 and JAB1 in acute myelogenous leukemia

摘要：目的 探讨硫氧还蛋白1(TRX1)和c-Jun激活区结合蛋白1(JAB1)在白血病各组中的表达，及TRX1水平在急性髓系白血病(AML)各组中的表达及其临床意义。方法 应用RT-PCR及Western blot的方法对白血病患者TRX1和JAB1的表达进行检测，比较各组之间的差异；进一步分析AML患者其基因表达阳性率与外周血白细胞计数的关系，并采用ELISA方法检测以上血清样本的TRX1水平。结果 在白血病患者中TRX1和JAB1基因和蛋白表达明显高于对照组(P<0.05)，相关分析显示AML初发和复发病例中TRX1和JAB1表达相关程度高，而且高表达的TRX1和JAB1与外周血白细胞计数有关(P<0.05)；ELISA检测外周血TRX1在AML的复发组高于初发组和对照组，初发组高于对照组，差异具有统计学意义(P<0.05)。结论 TRX1和JAB1之间呈正向关联，与AML的发生及病情进展有密切关系。
ABSTRACT: Objective To detect the expressions of thioredoxin (TRX1) and c-jun-activation-domain binding protein-1 (JAB1) in patients with acute myelogenous leukemia (AML) and healthy controls, and measure the TRX1 level in AML patients at different stages for evaluating its clinical significance. Methods The expressions of TRX1 and JAB1 in leukemia samples were analyzed by RT-PCR and Western blot at mRNA and protein levels, respectively. The correlation between TRX1 and JAB1, and the relationship between the gene expression and peripheral blood leukocytes count were also analyzed. Furthermore, serum TRX1 was measured by ELISA. Results TRX1 and JAB1 expressions at both mRNA and protein levels were obviously upregulated in leukemia patients (P<0.05). TRX1 was positively related to JAB1 in both newly diagnosed and recurrent AML patients. And high levels of TRX1 and JAB1 expressions were associated with white blood cell (WBC) counts in AML patients (P<0.05). Moreover, abundance of TRX1 in serum was significantly greater in AML patients, especially in the patients with recurrent AML, than in healthy donors (P<0.05). Conclusion There is a positive correlation between the expressions of TRX1 and JAB1, which is closely related to the occurrence and progression of AML