miR-199a-3p靶基因预测及生物信息学分析
Prediction of miR-199a-3p targets gene and its bioinformatics analysis

摘要：目的 为深入研究miR-199a-3p在膀胱癌形成和发展中的作用提供理论依据。方法 分析miR-199a-3p序列，预测其靶基因和转录因子，并对靶基因进行GO富集和KEGG Pathway分析；构建TF-miR-199a-3p-靶基因网络调控图。结果 miR-199a-3p序列在多物种间具有高度保守性；GO分析发现miR-199a-3p的靶基因参与细胞调节、代谢调节、细胞大分子生物合成等生物过程(P<0.01)；KEGG Pathway分析发现miR-199a-3p的靶基因显著富集在上皮细胞的细菌入侵通路、ECM受体的相互作用通路、PI3K-Akt信号通路、MAPK信号通路、小细胞肺癌通路、癌症中的蛋白聚糖通路等；根据构建的TF-miR-199a-3p-靶基因网络调控图，挖掘出可能受miR-199a-3p调控的重要基因有MYC、SP1、mTOR、NFκB、NFκB1等。结论 miR-199a-3p可能通过直接靶向作用mTOR，调节PI3K-Akt-mTOR信号通路，从而参与膀胱癌的形成和发展。
ABSTRACT:Objective To provide theoretical guidance for further research on the role of miR-199a-3p in formation and development of bladder cancer. Methods Mature sequence of miR-199a-3p was analyzed; target genes and transcription factors of miRNA-199a-3p were predicted, and the target genes were analyzed for gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway. Then TF-miRNA- mRNA network diagram was constructed. Results Sequences of miR-199a-3p were highly conserved in various species. In GO analysis, the target genes of miR-199a-3p were enriched in many biological processes, such as regulation of cellular process, regulation of macromolecule metabolic process, and regulation of biological process (P<0.01). In KEGG pathway, the target genes were mainly located in bacterial invasion pathway of epithelial cells, ECM-receptor interaction pathway, PI3K-Akt signaling pathway, MAPK signaling pathway, small cell lung cancer pathway, and proteoglycans pathway in the cancer (P<0.05). According to the TF-miRNA-mRNA network diagram, the important genes that might be regulated by miR-199a-3p were MYC, SP1, mTOR, NFκB, and NFκB1. Conclusion miR-199a-3p may directly target mTOR and participate in the formation and development of bladder cancer through regulating PI3K-Akt-mTOR signaling pathway