重度子痫前期胎盘中CD142的表达与其启动子区甲基化程度的关系
Relationship between expression of CD142 protein and the promoter methylation in the placenta in severe preeclampsia

摘要：目的 探究CD142在重度子痫前期孕妇胎盘中的表达情况及其与启动子区域甲基化之间的关系。方法 本研究采用实时荧光定量PCR、免疫组化、蛋白质印迹检测24例重度子痫前期孕妇和24例正常妊娠孕妇胎盘中CD142基因的表达情况；采用甲基化特异性PCR检测CD142启动子区域的甲基化情况。结果 病例组胎盘中CD142 mRNA相对含量(1.45±0.42)高于正常妊娠孕妇(0.25±0.28)，差异有统计学意义(P<0.05)；病例组胎盘中CD142蛋白表达量(0.857±0.043)高于正常晚孕者(0.248±0.035)，差异有统计学意义(P<0.05)；病例组胎盘组织中CD142启动子区域的甲基化阳性率低于对照组(29.2% vs. 100.0%，χ2＝36.11，P<0.001)，非甲基化阳性率高于正常组(100.0% vs. 20.8%，χ2＝29.85，P<0.001)；CD142的蛋白表达水平与其启动子区域的甲基化程度呈负相关(r=－0.909，P<0.05)。结论 CD142通过甲基化调控蛋白的表达在重度子痫前期的发病过程中发挥重要作用。
ABSTRACT: Objective To explore the relationship between expression of CD142 protein and the promoter methylation in the placenta of severe preeclampsia patients. Methods We assessed 24 patients complicated with severe preeclampsia as case group and 24 normal pregnant women as control group via qRT-PCR, immunohistochemistry and Western blotting for CD142 expression and MSP technology for methylation in CD142 promoter region. Results The relative expression quantity of CD142 mRNA in severe preeclampsia group (1.45±0.42) was higher than that in normal group (0.25±0.28)(P<0.05). The expression quantity of CD142 protein in severe preeclampsia group (0.857±0.043) was higher than that in normal group (0.248±0.035)(P<0.05). The positive rate of CD142 promoter region methylation was lower in severe preeclampsia group than in normal group (29.2% vs. 100.0%, χ2＝36.11, P<0.001) while the positive rate of CD142 promoter region unmethylation was higher than that in the latter group (100.0% vs. 20.8%, χ2＝29.85, P<0.001). A negative correlation was found between the CD142 promoter methylation level and the expression quantity of CD142 protein (r=－0.909, P<0.05). Conclusion The expression of CD142 protein regulated by the promoter methylation plays a crucial role in the mechanism of severe preeclampsia