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- 2017
miR-498通过下调FOXM1抑制肺腺癌细胞系A549的迁移侵袭
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Abstract:
摘要：目的 探索微小RNA498(miR-498)是否通过下调FOXM1抑制肺癌细胞系(A549)细胞的迁移和侵袭。方法 转染miR-498mimic和miR-NC至培养的A549细胞中，Western blot检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况，划痕试验和Transwell法观察细胞迁移侵袭情况，荧光素酶试验验证FOXM1是miR-498的靶基因；再通过转染过表达FOXM1的质粒至已成功转染miR-498的A549细胞中，检测A549细胞中COL1A1、COL1A5、FOXM1的表达情况，划痕试验和Transwell法观察细胞迁移侵袭情况。结果 与空质粒组比较，转染miR-498mimic的A549细胞中COL1A1、COL1A5、FOXM1的表达减少(P<0.05)，A549细胞的迁移侵袭减少(P<0.01)；荧光素酶实验结果显示，miR-498能够显著降低FOXM1-3′-UTR质粒的荧光素活性(P<0.05)；与miR-498+空质粒组比较，转染过表达FOXM1质粒的A549细胞中COL1A1、COL1A5表达增加(P<0.05)，细胞的迁移侵袭增加(P<0.01)。 结论 miR-498可以通过下调FOXM1而抑制A549的迁移和侵袭。
ABSTRACT: Objective To verify whether miR-498 can inhibit A549 cell migration and invasion by down-regulating FOXM1. Methods miR-498 mimic and miR-NC were transfected into A549 cells. Wound healing and Transwell method were employed to test the migratory ability and invasion ability of A549 cells; Western blot was used to detect the expressions of COL1A1, COL1A5 and FOXM1 in A549 cells. Luciferase assay was used to confirm whether FOXM1 is the target gene of miR-498. Results Compared with those in the control group, the expressions of COL1A1, COL1A5 and FOXM1 were decreased, and the migration and invasion abilities of A549 cells were decreased in the miR-498 group (both P<0.01). The luciferase activity of the FOXM1-3′-UTR plasmid was significantly suppressed by miR-498 (P<0.05); over-expression of FOXM1 could reverse the effect of miR-498 on A549 cells. Conclusion miR-498 inhibits A549 cell migration and invasion by down-regulating FOXM1
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