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- 2018
中药复方清化饮对慢性萎缩性胃炎大鼠血清IL-10、NO、GAS及血浆MTL的影响
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Abstract:
摘要：目的 探讨中药复方清化饮对大鼠慢性萎缩性胃炎(CAG)胃黏膜病理与血清白细胞介素-10(IL-10)、一氧化氮(NO)、胃泌素(GAS)及血浆胃动素(MTL)的影响。方法 将53只Wistar大鼠随机分为空白组8只和CAG造模组45只，予建立病证结合CAG大鼠模型。抽检大鼠确认造模成功后，将CAG造模组余下40只大鼠随机分为模型组、维酶素组、中药低、中、高剂量组各8只。予相应药物干预30d后处死大鼠，腹主动脉取血并留取胃组织，分析各组大鼠胃黏膜病理与血清IL-10、NO、GAS及血浆MTL浓度。结果 与空白组比较，模型组大鼠见不同程度的胃黏膜萎缩，血清IL-10、GAS浓度降低，血清NO、血浆MTL含量升高(P<0.01)。与模型组比较，清化饮可不同程度改善胃黏膜病理情况，升高血清IL-10、GAS浓度，降低血清NO、血浆MTL含量(P<0.05或P<0.01)，且高剂量组疗效更为显著(P<0.01)。结论 中药复方清化饮可调控血清IL-10、NO、GAS及血浆MTL的表达失衡，有效逆转CAG大鼠胃黏膜病变。
ABSTRACT: Objective To explore the effects of Chinese herbal compound Qinghuayin on the pathological changes of gastric mucosa and interleukin-10 (IL-10), nitric oxide (NO), gasmn (GAS) and motilin (MTL) in the serum in the animal model of chronic atrophic gastritis (CAG) in rats. Methods We divided 53 Wistar rats randomly into blank control group (n=8) and CAG model group (n=45), and the animal model of CAG in rats was replicated by combination of disease and syndrome. After confirming the sampled rat model was successful built, the other 40 CAG rats in CAG model group were divided into model group, vitacoenzyme tablet group, low-dosage TMC group, medium-dosage TMC group, and high-dosage TMC group (each group n=8). With the corresponding drug intervention to different rats for 30 days, the rats were executed. Then their blood was drawn from the abdominal aorta and the gastric tissue was taken to analyze the changes of serum IL-10, NO, GAS and MTL concentrations and gastric mucosa pathology. Results Compared with blank control group, model group had various degrees of gastric mucosa atrophy; decreased concentrations of serum IL-10 and GAS; increased NO and MTL (P<0.01). Compared with model group, Qinghuayin could improve gastric mucosa pathology in different degrees and increase the concentrations of IL-10 and GAS, decrease the concentrations of NO and MTL (P<0.05 or P<0.01). What’s more, the curative effect in high-dosage TMC group was better (P<0.01). Conclusion Chinese herbal compound Qinghuayin can effectively regulate the lopsided expressions of serum IL-10, NO, GAS and MTL and reverse the pathological and histological changes in the gastric mucosa of CAG rats
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