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- 2018
三七皂苷对激素性股骨头坏死早期细胞凋亡的影响及其机制
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Abstract:
摘要：目的 探讨三七皂苷(panax notoginseng saponins, PNS)对激素性股骨头坏死(steonecrosis of the femoral head, SONFH)早期细胞凋亡的影响及其作用机制。方法 选取健康成年新西兰兔24只，分为正常对照组、模型组及PNS组3组。模型组采用马血清(10mL/kg)加激素(40mg/kg)的方法建立兔SONFH模型；PNS组在模型组基础上同时给予PNS(50mg/kg)干预；正常对照组常规饲养。2周后处死动物取双侧股骨头进行脱钙包埋，TUNEL染色观察细胞内凋亡变化，BCA法检测股骨头内caspase-3活性变化，免疫组化及Western blot 检测各组股骨头内Bax及Bcl-2含量的变化。结果 TUNEL染色结果发现，模型组骨组织内可见大量凋亡细胞，PNS组骨组织内凋亡细胞明显减少(P<0.05)；与模型组相比，经PNS预处理后股骨头组织内的caspase-3活性明显下降(P<0.05)；免疫组化染色及Western blot检测结果证实，模型组骨组织内Bax蛋白表达量明显增加，而Bcl-2蛋白表达量明显减少，而与之相反的是PNS组骨组织内检测到的Bax蛋白表达量明显下降(P<0.05)，而Bcl-2蛋白表达量明显增加(P<0.05)。结论 PNS可能通过增强Bcl-2表达，降低Bax表达，抑制caspase-3活性，减少早期SONFH股骨头组织内的细胞凋亡。
ABSTRACT: Objective To investigate the effects of panax notoginseng saponins (PNS) on early-stage apoptosis in steonecrosis of rabbit femoral head. Methods The 24 rabbits were randomly divided into three groups: normal control group, model group, and PNS group. In model group, equine serum (10mL/kg) and methylprednisolone (40mg/kg) were injected alternatively to induce osteonecrosis of the femoral head. In PNS group, PNS (50mg/kg, i.v.) was administered continuously before methylprednisolone management. In control group, the rabbits were fed routinely. TUNEL method was used to detect apoptotic cells. Caspase-3 activity in the femoral head was measured by caspase-3 assay kit. Immunohistochemistry method and Western blot analysis were applied to detect the expressions of Bcl-2 and Bax. Results In model group, typical appearance of apoptotic cells was observed in the femoral head by TUNEL staining (P<0.05). PNS treatment significantly inhibited cell apoptosis of the femoral head in model group. Furthermore, pretreatment with PNS significantly reversed the inhibition of Bcl-2 expression, augmentation of Bax expression and caspase-3 activity (P<0.05). Conclusion PNS could up-regulate the expression of Bcl-2, down-regulate that of Bax, reduce the activity of caspase-3, and inhibit cell apoptosis of the femoral head
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