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- 2016
ALOX15基因单核苷酸多态性与冠心病遗传易感性的相关性
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Abstract:
摘要：目的 探讨中国陕西地区汉族人群花生四烯酸15-脂加氧酶(arachidonate 15-lipoxygenase, ALOX15)基因多态性是否是冠心病(coronary heart disease, CHD)的遗传易感因素，从而为该病的早期诊断和预防提供相关依据。方法 应用聚基质辅助激光解吸附电离飞行时间质谱方法(matrix-assisted laser desorption /ionization-time of flight, MALDI-TOF)，检测105例CHD患者(CHD组)和75例年龄及性别相匹配的非冠心病者(对照组)的ALOX15基因3个位点rs916055、rs2619112、rs2664593的单核苷酸多态性(single nucleotide polymorphisms, SNPs)，判定其基因型并统计各基因型及等位基因的频率。结果 rs916055A/G的基因型以及等位基因频率在CHD组和对照组间有统计学差异(P=0.0001，P=0.0001)；rs2619112A/G的基因型以及等位基因频率在CHD组和对照组间无统计学差异(P=0.1342，P=0.1438)；rs2664593C/G的基因型在CHD组和对照组间有统计学差异(P=0.0027)，等位基因频率在CHD组和对照组间无统计学差异(P=0.5371)；应用Logistic回归分析调整了其他相关因素后显示，rs916055位点携带A等位基因为CHD发病的独立危险因素。结论 rs916055位点可能与CHD发病相关，A等位基因可能是CHD的遗传易感基因。
ABSTRACT: Objective To investigate the correlation between arachidonate 15-lipoxygenase (ALOX15) gene polymorphism and its genetic predisposition to coronary heart disease (CHD) in Han population of Shaanxi Province so as to provide the basis for early diagnosis and prophylaxis of CHD. Methods The single nucleotide polymorphisms (SNPs) of ALOX15’s rs916055, rs2619112, and rs2664593 were measured by using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) method in 105 CHD patients (CHD group) and 75 non-CHD patients (control group) who were matched in age and sex. Results The frequencies of genotypes and alleles of SNPs rs916055A/G in CHD group were significantly different from those in control group (P=0.0001, P=0.0001). The frequencies of genotypes and alleles of SNP rs2619112A/G in CHD group did not significantly differ from those in control group (P=0.1342, P=0.1438). The frequencies of genotypes of SNP rs2664593C/G in CHD group significantly differed from those in control group (P=0.0027), but the frequencies of alleles were not significantly different (P=0.5371). Logistic regression analysis indicated that the A allele of SNP rs916055 was an independent risk factor for CHD. Conclusion SNP rs916055 may be related to CHD and its A allele may be the genetic susceptibility gene for CHD
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